Bovine Brain-derived neurotrophic factor ELISA Kit | BDNF elisa kit

Bovine Brain-derived neurotrophic factor (BDNF) ELISA Kit

Cat. Num. AAA13281

• Reactivity: Bovine
• Synonyms: Brain-derived neurotrophic factor; N/A; Bovine Brain-derived neurotrophic factor (BDNF) ELISA Kit; Neurotrophin; Abrineurin; BDNF elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine BDNF. No significant cross-reactivity or interference between Bovine BDNF and analogues was observed.
• Sequence Length: 246
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 0.156 ng/mL - 10 ng/mL
• Sensitivity: 0.1 ng/mL (mean of 6 independent assays)

• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for BDNF elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate BDNF in Bovine serum, plasma. An antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Intended Uses: For the quantitative detection of Bovine Brain-derived neurotrophic factor (BDNF) concentration in serum, plasma and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 114052963
• NCBI GeneID: 617701
• NCBI Accession #: NP_001040072.1
• NCBI GenBank Nucleotide #: NM_001046607.2
• UniProt Accession #: Q95106; Q32KY2
• Molecular Weight: 28,174 Da
• NCBI Official Full Name: brain-derived neurotrophic factor
• NCBI Official Synonym Full Names: brain derived neurotrophic factor
• NCBI Official Symbol: BDNF
• NCBI Protein Information: brain-derived neurotrophic factor
• UniProt Protein Name: Brain-derived neurotrophic factor
• UniProt Gene Name: BDNF
• UniProt Synonym Gene Names: BDNF