SDS-PAGE
BHMT (Betaine-homocysteine methyltransferase) Recombinant Protein | BHMT recombinant protein
BHMT (Betaine-homocysteine methyltransferase), 1-406 aa, Human, His tag, E Coli
Gene Names
BHMT; BHMT1; HEL-S-61p
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
BHMT (Betaine-homocysteine methyltransferase); N/A; BHMT (Betaine-homocysteine methyltransferase), 1-406 aa, Human, His tag, E Coli; Betaine-homocysteine methyltransferase; BHMT; EC 2.1.1.5; Betaine--homocysteine S-methyltransferase 1; Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase; Betaine homocysteine S methyltransferase; Betaine homocysteine S methyltransferase 1; BHMT1,; BHMT recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris pH7.5, 20% glycerol.
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP VGGKKAKKGI LERLNAGEIV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFID LPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPP ASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM QQKEATTEQQ LKELFEKQKF KSQ
Sequence Length
406
Applicable Applications for BHMT recombinant protein
SDS-PAGE
Tag Information
His-tag
Preparation and Storage
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for BHMT recombinant protein
Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis. Recombinant human BHMT, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for BHMT recombinant protein
References
Nandita, et al., Protein Expr Purif (2002) 25:73-80; Breksa, A P et al., Biochemistry (1999) 38:13991-8
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49.2 kDa (443 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
betaine--homocysteine S-methyltransferase 1
NCBI Official Synonym Full Names
betaine--homocysteine S-methyltransferase
NCBI Official Symbol
BHMT
NCBI Official Synonym Symbols
BHMT1; HEL-S-61p
NCBI Protein Information
betaine--homocysteine S-methyltransferase 1
UniProt Protein Name
Betaine--homocysteine S-methyltransferase 1
UniProt Gene Name
BHMT
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Product Notes
The BHMT bhmt (Catalog #AAA48469) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's BHMT (Betaine-homocysteine methyltransferase) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the BHMT bhmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP VGGKKAKKGI LERLNAGEIV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFID LPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPP ASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM QQKEATTEQQ LKELFEKQKF KSQ. It is sometimes possible for the material contained within the vial of "BHMT (Betaine-homocysteine methyltransferase), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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