Human Betaine Homocysteine Methyltransferase (BHMT) ELISA Kit | BHMT elisa kit

Human Betaine Homocysteine Methyltransferase (BHMT) ELISA Kit

Cat. Num. AAA187091

• Gene Names: BHMT; BHMT1; HEL-S-61p
• Reactivity: Human
• Synonyms: Betaine Homocysteine Methyltransferase (BHMT); N/A; Human Betaine Homocysteine Methyltransferase (BHMT) ELISA Kit; Betaine—homocysteine S-methyltransferase; BHMT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Betaine Homocysteine Methyltransferase (BHMT).; No significant cross-reactivity or interference between Betaine Homocysteine Methyltransferase (BHMT) and analogues was observed.
• Sequence Length: 406
• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Betaine Homocysteine Methyltransferase (BHMT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Betaine Homocysteine Methyltransferase (BHMT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for BHMT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37222747
• NCBI GeneID: 635
• UniProt Accession #: Q93088; Q9UNI9
• Molecular Weight: 44,998 Da
• NCBI Official Full Name: betaine homocysteine methyltransferase, partial
• NCBI Official Synonym Full Names: betaine--homocysteine S-methyltransferase
• NCBI Official Symbol: BHMT
• NCBI Official Synonym Symbols: BHMT1; HEL-S-61p
• NCBI Protein Information: betaine--homocysteine S-methyltransferase 1
• UniProt Protein Name: Betaine--homocysteine S-methyltransferase 1
• UniProt Gene Name: BHMT