beta-Amyloid Biochemical

beta-Amyloid (1-40), FAM-labeled

Cat. Num. AAA227857

• Purity: 98%
• Synonyms: beta-Amyloid; N/A; beta-Amyloid (1-40), FAM-labeled; beta-Amyloid biochemical

• Purity/Purification: 98%

• CAS: 1678416-08-4
• Solubility: <1mg/ml refers to the product slightly soluble or insoluble
• In vitro: Apical-to-basolateral exchange across endothelial monolayer of fluorescent Abeta42 (FAM-labeled Human beta-Amyloid (1-42) and Fluor 488-labeled beta-Amyloid (1-42)) or scramble Abeta42 (FAM-labeled scrambled beta-Amyloid (1-42))(1-100 uM) is monitored in transendothelial electrical resistance (TEER) during cell monolayer's formation over time (over 120 min in presence or absence of Abeta24). Human beta-Amyloid (1-24)(1 uM) results in H-Abeta42 retention, thus reducing its efflux through the BBB and therefore preventing an efficient mechanism of Abeta42 clearance[1].The in vitro BBB model is used to investigate the passage of FAM-labeled or 488-conjugated H-Abeta42 across the endothelial cell monolayer. The addition of fluorescently labeled H-Abeta42 (FAM-labeled Human beta-Amyloid (1-42) or Fluor 488-labeled beta-Amyloid (1-42)) to the apical side of cell inserts results in effective BBB crossing, FAM-labeled scrambled beta-Amyloid (1-42) is not efficiently transcytosed.
• Preparation and Storage: Powder: -20 degree C for 3 years; In solvent: -80 degree C for 2 years

Structure

(Propargyl-PEG5-PFP ester, CAS 2148295-92-3)

Structure

(Propargyl-PEG5-PFP ester, CAS 2148295-92-3)

Related Product Information for beta-Amyloid biochemical

beta-Amyloid (1-40), FAM-labeled is a FAM fluorescently-labelled beta-Amyloid (1-40) peptide (lambda ex = 492 nm and lambda em = 518 nm).

References

1. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562