IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Amyloid Beta 21F12 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of 21F12 (AAA72313) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72313, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human Amyloid Beta Antibody | anti-APP antibody
Anti-Amyloid Beta [21F12]
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation
Purity
Purified. Protein A affinity purified
Synonyms
Amyloid Beta; N/A; Anti-Amyloid Beta [21F12]; Abeta; A-beta; Abeta42; Alzheimer disease amyloid protein; S-APP-beta; anti-APP antibody
Reactivity
Human
Isotype
Rabbit IgG kappa
Clone Number
21F12
Specificity
This antibody recognizes amino acids 33-42 of amyloid beta.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-APP antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, IP (Immunoprecipitation)
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Amyloid Beta 21F12 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of 21F12 (AAA72313) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72313, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Amyloid Beta 21F12 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of 21F12 (AAA72313) (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72313, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-APP antibody
The original antibody was generated by immunizing A/J mice with a synthetic peptide (NH2-CYS-NH-CH2-(CH2)5-CO-GLMVGGVVIA-COOH) as an immunogen.
Describes the generation of different amino acid forms of amyloid beta by different protease activities.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Describes the generation of different amino acid forms of amyloid beta by different protease activities.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for anti-APP antibody
References
Citron et al.
Proc Natl Acad Sci U S A. (1996); 93(23):13170-5.
Proc Natl Acad Sci U S A. (1996); 93(23):13170-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
amyloid beta A4 protein isoform a
NCBI Official Synonym Full Names
amyloid beta (A4) precursor protein
NCBI Official Symbol
APP
NCBI Official Synonym Symbols
AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
NCBI Protein Information
amyloid beta A4 protein; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide
UniProt Protein Name
Amyloid beta A4 protein
UniProt Gene Name
APP
UniProt Synonym Gene Names
A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
UniProt Entry Name
A4_HUMAN
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Product Notes
The APP app (Catalog #AAA72313) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Amyloid Beta [21F12] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Amyloid Beta can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, IP (Immunoprecipitation). Researchers should empirically determine the suitability of the APP app for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Amyloid Beta, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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