NHC-diphosphate triammonium Biochemical

NHC-diphosphate triammonium

Cat. Num. AAA227226

• Purity: 98%
• Synonyms: NHC-diphosphate triammonium; N/A; NHC-diphosphate triammonium biochemical

• Purity/Purification: 98%

• Solubility: H2O: 160 mg/mL (340.23 mM),; Need ultrasonic and warming; <1mg/ml refers to the product slightly soluble or insoluble
• In vitro: In an intracellular metabolism assay, HCV replicon cells are treated with 10 uM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].NHC-triphosphate triammonium (NHC-TP)(5-40 uM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].Huh-7 cells are incubated with (10-50 uM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate triammonium remains the most abundant metabolite[2].NHC-triphosphate triammonium (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.
• Preparation and Storage: Powder: -20 degree C for 3 years; In solvent: -80 degree C for 2 years

Structure

(Amylin (8-37), human, CAS 135702-23-7)

Structure

(Amylin (8-37), human, CAS 135702-23-7)

Related Product Information for NHC-diphosphate triammonium biochemical

NHC-triphosphate triammonium is an active phosphorylated intracellular metabolite of beta-d-N4-Hydroxycytidine (NHC) as a triphosphate form[1]. NHC-triphosphate triammonium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA[1][2].

References

1. H C Champion, et al. Analysis of responses to hAmylin, hCGRP, and hADM in isolated resistance arteries from the mesenteric vascular bed of the rat. Peptides. 2001 Sep;22(9):1427-34.