TPC2-A1-P biochemical

TPC2-A1-P

Cat. Num. AAA227214

• Purity: 98%
• Synonyms: TPC2-A1-P; N/A; TPC2-A1-P biochemical

• Purity/Purification: 98%

• Solubility: DMSO: 50 mg/mL (108.63 mM),; Need ultrasonic; <1mg/ml refers to the product slightly soluble or insoluble
• In vitro: Two-pore channels (TPC1-3) are ancient members of the voltage-gated ion channel superfamily. TPCs are expressed throughout the endo-lysosomal system and regulates the trafficking of various cargoes[1].TPC2 can mediate different physiological and possibly pathophysiological effects depending on how it is activated. The ion selectivity of TPC2 is not fixed but rather agonist-dependent. TPC2 is a unique example of an ion channel that conducts different ions in response to different activating ligands[1].TPC2-A1-P (10 uM) reproducibly evokes Ca2+ signals, and TPC2-A1-P response reachs its plateau slower than TPC2-A1-N. The EC50 in full concentration-effect relationships for the plateau response is 10.5 uM for TPC2-A1-P in a cell line stably expressing TPC2L11A/L12A.TPC2-A1-P (10-30 uM) induces Ca2+ signals in Hela cells expressing TPC2 in the presence but not absence of extracellular Ca2+. However, the responses are smaller and delayed compared to TPC2-A1-N, consistent with the results obtained in cells stably expressing TPC2L11A/L12A. TPC2-A1-P fails to induce Ca2+ signals in cells expressing 'pore-dead' TPC2L11A/L12A/L265P and also fails to evoke Ca2+ signals in cells expressing human TRPML1 re-routed to the plasma membrane (TRPML1deltaNC)[1].In endo-lysosomal patch-clamp experiments, TPC2-A1-P (10 uM) evokes currents in endo-lysosomes isolated from cells expressing TPC2 and TPC2M484L, the currents evoked by TPC2-A1-P are significantly larger than those evoked by TPC2-A1-N in both wild-type and gain-of-function variant,and exhibits an EC50 value of 0.6 uM for TPC2-A1-P[1].
• Preparation and Storage: Powder: -20 degree C for 3 years; In solvent: -80 degree C for 2 years

Structure

(Fmoc-Val-D-Cit-PAB, CAS 1350456-67-5)

Structure

(Fmoc-Val-D-Cit-PAB, CAS 1350456-67-5)

Related Product Information for TPC2-A1-P biochemical

TPC2-A1-P is a powerful and membrane permeable agonist of two pore channel 2 (TPC2) with an EC50 of 10.5 uM. TPC2-A1-P plays its role by mimicking the physiological actions of PI(3,5)P2. TPC2-A1-P also shows higher potency to induce Na2+ mobilisation from TPC2 than TPC-A1-N. TPC2-A1-P can be used to probe different functions of TPC2 channels in intact cells[1][2][3].

References

1. Mondal D, et al. Improved Methodology for the Synthesis of a Cathepsin B Cleavable Dipeptide Linker, Widely Used in Antibody-Drug Conjugate Research. Tetrahedron Lett. 2018;59(40):3594-3599.