Canine B-Cell Linker Protein ELISA Kit | BLNK elisa kit

Canine B-Cell Linker Protein ELISA Kit

Cat. Num. AAA94558

• Gene Names: BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• Reactivity: Canine
• Synonyms: B-Cell Linker Protein; N/A; Canine B-Cell Linker Protein ELISA Kit; BLNK elisa kit

• Reactivity: Canine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL

• Intended Uses: This BLNK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BLNK. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for BLNK elisa kit

Principle of the assay: BLNK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BLNK antibody and an BLNK-HRP conjugate. The assay sample and buffer are incubated together with BLNK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BLNK concentration since BLNK from samples and BLNK-HRP conjugate compete for the anti-BLNK antibody binding site. Since the number of sites is limited, as more sites are occupied by BLNK from the sample, fewer sites are left to bind BLNK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BLNK concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BLNK elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 166235901
• NCBI GeneID: 29760
• NCBI Accession #: NP_001107566.1
• NCBI GenBank Nucleotide #: NM_001114094.1
• Molecular Weight: 44,451 Da
• NCBI Official Full Name: B-cell linker protein isoform 2
• NCBI Official Synonym Full Names: B-cell linker
• NCBI Official Symbol: BLNK
• NCBI Official Synonym Symbols: bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
• NCBI Protein Information: B-cell linker protein; B-cell activation; B cell linker protein; cytoplasmic adapter protein; B cell adaptor containing SH2 domain; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Src homology 2 domain-containing leukocyte protein of 65 kDa
• UniProt Protein Name: B-cell linker protein
• UniProt Gene Name: BLNK
• UniProt Synonym Gene Names: BASH; SLP65; SLP-65
• UniProt Entry Name: BLNK_HUMAN