Bovine A disintegrin and metalloproteinase with thrombospondin motifs 10 (ADAMTS10) ELISA Kit | ADAMTS10 elisa kit

Bovine A disintegrin and metalloproteinase with thrombospondin motifs 10 (ADAMTS10) ELISA Kit

Cat. Num. AAA244998

• Gene Names: ADAMTS10; WMS; WMS1; ADAM-TS10; ADAMTS-10
• Reactivity: Bovine
• Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 10 (ADAMTS10); N/A; Bovine A disintegrin and metalloproteinase with thrombospondin motifs 10 (ADAMTS10) ELISA Kit; ADAMTS10 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADAMTS10. No significant cross-reactivity or interference between ADAMTS10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTS10 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ADAMTS10 elisa kit

Principle of the Assay: ADAMTS10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADAMTS10 antibody and an ADAMTS10-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS10 concentration since ADAMTS10 from samples and ADAMTS10-HRP conjugate compete for the anti-ADAMTS10 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS10 from the sample, fewer sites are left to bind ADAMTS10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS10 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ADAMTS10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ADAMTS10. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for ADAMTS10 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 537544566
• NCBI GeneID: 81794
• NCBI Accession #: NP_112219.3
• NCBI GenBank Nucleotide #: NM_030957.3
• Molecular Weight: 62,530 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 10
• NCBI Official Symbol: ADAMTS10
• NCBI Official Synonym Symbols: WMS; WMS1; ADAM-TS10; ADAMTS-10
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 10; ADAM-TS 10; zinc metalloendopeptidase; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 10
• UniProt Gene Name: ADAMTS10
• UniProt Synonym Gene Names: ADAM-TS 10; ADAM-TS10; ADAMTS-10
• UniProt Entry Name: ATS10_HUMAN