Bovine CD83 antigen (CD83) ELISA Kit | CD83 elisa kit
Bovine CD83 antigen (CD83) ELISA Kit
Gene Names
CD83; BL11; HB15
Reactivity
Bovine
Synonyms
CD83 antigen (CD83); N/A; Bovine CD83 antigen (CD83) ELISA Kit; CD83 elisa kit
Reactivity
Bovine
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CD83 elisa kit
Intended Uses: This CD83 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CD83. This ELISA kit for research use only!
Principle of the Assay||CD83 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD83 antibody and an CD83-HRP conjugate. The assay sample and buffer are incubated together with CD83-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD83 concentration since CD83 from samples and CD83-HRP conjugate compete for the anti-CD83 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD83 from the sample, fewer sites are left to bind CD83-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD83 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||CD83 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD83 antibody and an CD83-HRP conjugate. The assay sample and buffer are incubated together with CD83-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD83 concentration since CD83 from samples and CD83-HRP conjugate compete for the anti-CD83 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD83 from the sample, fewer sites are left to bind CD83-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD83 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CD83 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,042 Da
NCBI Official Full Name
CD83 antigen isoform a
NCBI Official Synonym Full Names
CD83 molecule
NCBI Official Symbol
CD83
NCBI Official Synonym Symbols
BL11; HB15
NCBI Protein Information
CD83 antigen; hCD83; B-cell activation protein; cell surface protein HB15; cell-surface glycoprotein; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
UniProt Protein Name
CD83 antigen
UniProt Gene Name
CD83
UniProt Synonym Gene Names
hCD83
UniProt Entry Name
CD83_HUMAN
