Application Data
(HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
BPTF/FALZ recombinant protein
Recombinant BPTF/FALZ (2791-2911) protein
Gene Names
BPTF; FAC1; FALZ; NURF301
Purity
The purity ofThe protein is >90% by SDS-PAGE.
Synonyms
BPTF/FALZ; N/A; Recombinant BPTF/FALZ (2791-2911) protein; FAC1; NURF301; BPTF/FALZ recombinant protein
Host
E Coli
Purity/Purification
The purity ofThe protein is >90% by SDS-PAGE.
Form/Format
Recombinant BPTF/FALZ (2791-2911) protein is supplied in 25mM Tris pH8.0, 500mM NaCl, 20% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant BPTF/FALZ (2791-2911) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Application Data
(HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Application Data
(HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Application Data
(HTRF Assay for Recombinant BPTF / FALZ (2791-2911) activity. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
SDS-PAGE
(Recombinant BPTF / FALZ (2791-2911) protein gel. BPTF / FALZ (2791-2911) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 20.3 kDa Purity: > 90%)
SDS-PAGE
(Recombinant BPTF / FALZ (2791-2911) protein gel. BPTF / FALZ (2791-2911) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 20.3 kDa Purity: > 90%)
Related Product Information for BPTF/FALZ recombinant protein
Short Description: The peptide corresponding to amino acids 2791-2911 that contains the bromodomain sequences of BPTF/FALZ (accession number NP_872579.2) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 20.3 kDa. Bromodomain PHD finger transcription factor (BPTF), also known as Fetal Alzheimer antigen (FALZ), is a histone-binding component of NuRF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. BPTF protein contains a DNA-binding domain, a zinc finger motif and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. BPTF also specifically recognizes H3 tails trimethylated on lysine 4 (H3K4me3), which mark transcription start sites of virtually all active genes. It may also regulate transcription through direct binding to DNA or transcription factors. Recombinant BPTF/FALZ (2791-2911) can be used in binding assays and inhibitor screening.
Background: BPTF (Bromodomain PHD finger transcription factor) is a histone-binding component of NuRF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. BPTF protein contains a DNA-binding domain, a zinc finger motif and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. BPTF also specifically recognizes H3 tails trimethylated on lysine 4 (H3K4me3), which mark transcription start sites of virtually all active genes. It may also regulate transcription through direct binding to DNA or transcription factors.
Background: BPTF (Bromodomain PHD finger transcription factor) is a histone-binding component of NuRF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. BPTF protein contains a DNA-binding domain, a zinc finger motif and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. BPTF also specifically recognizes H3 tails trimethylated on lysine 4 (H3K4me3), which mark transcription start sites of virtually all active genes. It may also regulate transcription through direct binding to DNA or transcription factors.
Product Categories/Family for BPTF/FALZ recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
322,217 Da
NCBI Official Full Name
Homo sapiens bromodomain PHD finger transcription factor (BPTF), transcript variant 1, mRNA
NCBI Official Synonym Full Names
bromodomain PHD finger transcription factor
NCBI Official Symbol
BPTF
NCBI Official Synonym Symbols
FAC1; FALZ; NURF301
NCBI Protein Information
nucleosome-remodeling factor subunit BPTF; bromodomain and PHD domain transcription factor; bromodomain and PHD finger-containing transcription factor; fetal Alz-50 clone 1 protein; fetal Alz-50 reactive clone 1; fetal Alzheimer antigen; nucleosome remode
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Product Notes
The BPTF/FALZ (Catalog #AAA59689) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BPTF/FALZ, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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