Purity Info
(The purity of BRAF (G466VV) protein was determined to be >80% by densitometry, approx. MW is 69 kDa)
BRAF recombinant protein
BRAF Mutant (G466V) Protein
Gene Names
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Applications
Kinase Assay
Purity
>80%
Synonyms
BRAF; N/A; BRAF Mutant (G466V) Protein; BRAF (G466V) Protein; BRAF recombinant protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>80%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1mg/ml (varies by lot)
Sequence Positions
381-end or exon 9-18 (G466V)
Sequence Length
2949
Applicable Applications for BRAF recombinant protein
Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity Info
(The purity of BRAF (G466VV) protein was determined to be >80% by densitometry, approx. MW is 69 kDa)
Purity Info
(The purity of BRAF (G466VV) protein was determined to be >80% by densitometry, approx. MW is 69 kDa)
Specific Activity
(BRAF (G466V) activity assay with or without downstream substarte unactive MEK-ERK1. THis recombinant BRAF mutant did not show significant kinase activity in vitro using the activity assay protocol in the datasheet.)
Specific Activity
(BRAF (G466V) activity assay with or without downstream substarte unactive MEK-ERK1. THis recombinant BRAF mutant did not show significant kinase activity in vitro using the activity assay protocol in the datasheet.)
Related Product Information for BRAF recombinant protein
Description: Recombinant human BRAF (G466V) (381-end or exon 9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF (2).
Scientific Background: BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF (2).
Product Categories/Family for BRAF recombinant protein
References
1. Weber, C K. et al: Active Ras induces heterodimerization of cRaf and BRaf. Cancer Res. 2001 May 1;61 (9):3595-8. 2. Mercer, K E. et al: Raf proteins and cancer: B-Raf is identified as a mutational target. Biochim Biophys Acta. 2003 Jun 5;1653 (1):25-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~69 kDa
NCBI Official Full Name
Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
BRAF
NCBI Official Synonym Symbols
NS7; B-raf; BRAF1; RAFB1; B-RAF1
NCBI Protein Information
serine/threonine-protein kinase B-raf
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Gene Name
BRAF
UniProt Synonym Gene Names
BRAF1; RAFB1
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Product Notes
The BRAF braf (Catalog #AAA72953) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 381-end or exon 9-18 (G466V). AAA Biotech's BRAF can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the BRAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRAF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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