Application Data
(All lanes: Anti B Raf (AAA46060) at 0.5ug/mlLane 1: Rat Testis Tissue at 50ugLane 2: Rat Brain Tissue at 50ugLane 3: Mouse Testis Tissue at 50ugLane 4: Mouse Brain Tissue at 50ugLane 5: Hela Whole Cell Lysate at 40ugLane 6: Jurkat Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugLane 8: K562 Whole Cell Lysate at 40ugPredicted bind size: 84KDObserved bind size: 84KD)
Rabbit Serine/threonine-protein kinase B-raf Polyclonal Antibody | anti-BRAF antibody
Anti-B Raf Antibody
Gene Names
BRAF; NS7; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Serine/threonine-protein kinase B-raf, Antibody; Anti-B Raf Antibody; FLJ95109 antibody; 94 kDa B raf protein antibody; B raf 1 antibody; B Raf proto oncogene serine threonine protein kinase antibody; B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody; BRAF 1 antibody; BRaf antibody; BRAF_HUMAN antibody; BRAF1 antibody; cRmil antibody; MGC126806 antibody; MGC138284 antibody; Murine sarcoma viral (v-raf) oncogene homolog B1 antibody; Murine sarcoma viral v raf oncogene homolog B1 antibody; NS7 antibody; oncogene BRAF1 antibody; p94 antibody; Proto-oncogene B-Raf antibody; Proto-oncogene c-Rmil antibody; RAFB 1 antibody; RAFB1 antibody; RMIL antibody; Serine/threonine-protein kinase B-raf antibody; v raf murine sarcoma viral oncogene homolog B antibody; v raf murine sarcoma viral oncogene homolog B1 antibody; v-Raf murine sarcoma viral oncogene homolog B1 antibody; B-Raf proto-oncogene, serine/threonine kinase; anti-BRAF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
766
Applicable Applications for anti-BRAF antibody
WB (Western Blot)
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Application Data
(All lanes: Anti B Raf (AAA46060) at 0.5ug/mlLane 1: Rat Testis Tissue at 50ugLane 2: Rat Brain Tissue at 50ugLane 3: Mouse Testis Tissue at 50ugLane 4: Mouse Brain Tissue at 50ugLane 5: Hela Whole Cell Lysate at 40ugLane 6: Jurkat Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugLane 8: K562 Whole Cell Lysate at 40ugPredicted bind size: 84KDObserved bind size: 84KD)
Application Data
(All lanes: Anti B Raf (AAA46060) at 0.5ug/mlLane 1: Rat Testis Tissue at 50ugLane 2: Rat Brain Tissue at 50ugLane 3: Mouse Testis Tissue at 50ugLane 4: Mouse Brain Tissue at 50ugLane 5: Hela Whole Cell Lysate at 40ugLane 6: Jurkat Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugLane 8: K562 Whole Cell Lysate at 40ugPredicted bind size: 84KDObserved bind size: 84KD)
Application Data
(All lanes: Anti B Raf (AAA46060) at 0.5ug/mlWB: Recombinant Human B Raf Protein 0.5ngPredicted bind size: 40KDObserved bind size: 40KD)
Application Data
(All lanes: Anti B Raf (AAA46060) at 0.5ug/mlWB: Recombinant Human B Raf Protein 0.5ngPredicted bind size: 40KDObserved bind size: 40KD)
Related Product Information for anti-BRAF antibody
Description: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human;Mouse;Rat.
Background: BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.
Background: BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,437 Da
NCBI Official Full Name
serine/threonine-protein kinase B-raf
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
BRAF
NCBI Official Synonym Symbols
NS7; BRAF1; RAFB1; B-RAF1
NCBI Protein Information
serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94)
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Gene Name
BRAF
UniProt Synonym Gene Names
BRAF1; RAFB1
UniProt Entry Name
BRAF_HUMAN
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Product Notes
The BRAF braf (Catalog #AAA46060) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-B Raf Antibody reacts with Human, Mouse, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Serine/threonine-protein kinase B-raf can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the BRAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serine/threonine-protein kinase B-raf, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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