Standard Curve (Sample)
Human CD147 ELISA Kit | BSG elisa kit
Human CD147 ELISA Kit
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Human
Applications
ELISA
Synonyms
CD147; N/A; Human CD147 ELISA Kit; Basigin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; BSG elisa kit
Reactivity
Human
Specificity
The Human CD147 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD147 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Sequence Length
385
Samples
Culture Supernatants, Serum, Plasma, Urine
Sensitivity
< 2 pg/ml
Applicable Applications for BSG elisa kit
ELISA (Sandwich ELISA)
Detection Method
Colorimetric
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for BSG elisa kit
Background: Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.
Principle of the Assay: The Human CD147 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD147 in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human CD147 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD147 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD147 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD147 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Human CD147 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD147 in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human CD147 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD147 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD147 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD147 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,764 Da
NCBI Official Full Name
basigin isoform 1
NCBI Official Synonym Full Names
basigin (Ok blood group)
NCBI Official Symbol
BSG
NCBI Official Synonym Symbols
M6; OK; 5F7; TCSF; CD147; EMMPRIN
NCBI Protein Information
basigin; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagenase stimulatory factor
UniProt Protein Name
Basigin
UniProt Gene Name
BSG
UniProt Synonym Gene Names
EMMPRIN; TCSF
UniProt Entry Name
BASI_HUMAN
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Product Notes
The Human BSG bsg (Catalog #AAA105706) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA105706 ELISA Kit recognizes Human BSG. AAA Biotech's CD147 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the BSG bsg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD147, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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