Human Emmprin ELISA Kit | BSG elisa kit

Human Emmprin PicoKine ELISA Kit

Cat. Num. AAA45263

• Gene Names: BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
• Reactivity: Human
• Synonyms: Emmprin; N/A; Human Emmprin PicoKine ELISA Kit; Basigin; basigin (Ok blood group); 5F7; BASI_HUMAN; Bsg; CD147; Collagenase stimulatory factor; EMMPRIN; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; OK blood group antigen; TCSF; Tumor cell-derived collagenase stimulatory factor; BSG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Emmprin
• Sequence Length: 385
• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 2pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human Emmprin PicoKine ELISA Kit standard curve)

Related Product Information for BSG elisa kit

Principle of the assay: human Emmprin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Emmprin has been precoated onto 96-well plates. Standards(NSO, T25-H205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Emmprin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Emmprin amount of sample captured in plate.
Background: Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147).[1][2] It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.[3][4][5] It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 51704273
• NCBI GeneID: 682
• UniProt Accession #: P35613; Q7Z796; Q8IZL7; A6NJW1; D3YLG5
• Molecular Weight: 22,764 Da
• NCBI Official Full Name: Basigin
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: M6; OK; 5F7; TCSF; CD147; EMMPRIN
• NCBI Protein Information: basigin; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagenase stimulatory factor
• UniProt Protein Name: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: EMMPRIN; TCSF
• UniProt Entry Name: BASI_HUMAN