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product-image-AAA112578_SC15.png Standard Curve (Sample)

Human complement component 4 binding protein, beta ELISA Kit | C4BPB elisa kit

Human C4b-binding protein beta chain, C4BPB ELISA Kit

Gene Names
C4BPB; C4BP
Reactivity
Human
Synonyms
complement component 4 binding protein, beta; N/A; Human C4b-binding protein beta chain, C4BPB ELISA Kit; Human C4b-binding protein beta chain (C4BPB) ELISA kit; C4BP; C4b binding protein; beta chain; complement component 4 binding protein; complement component 4-binding protein; beta; C4BPB elisa kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human C4BPB. No significant cross-reactivity or interference between human C4BPB and analogues was observed.
Sequence Length
252
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
3.12 ng/ml -200 ng/ml.
Sensitivity
The minimum detectable dose of human C4BPB is typically less than 0.78 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

product-image-AAA112578_SC15.png Standard Curve (Sample)
Related Product Information for C4BPB elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4BPB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4BPB present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for C4BPB is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4BPB bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
725
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,286 Da
NCBI Official Full Name
C4b-binding protein beta chain isoform 1
NCBI Official Synonym Full Names
complement component 4 binding protein, beta
NCBI Official Symbol
C4BPB
NCBI Official Synonym Symbols
C4BP
NCBI Protein Information
C4b-binding protein beta chain
UniProt Protein Name
C4b-binding protein beta chain
UniProt Gene Name
C4BPB
UniProt Entry Name
C4BPB_HUMAN

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Product Notes

The Human C4BPB c4bpb (Catalog #AAA112578) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112578 ELISA Kit recognizes Human C4BPB. It is sometimes possible for the material contained within the vial of "complement component 4 binding protein, beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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