Application Data
(HTRF assay for CAMK2D activity 1uM STK S1 substrate was incubated with different concentrations of CAMK2D protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 500uM CaCl2,1uM Calmodulin, 1 mM DTT, 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
CAMK2D recombinant protein
Recombinant CAMK2D protein
Synonyms
CAMK2D; N/A; Recombinant CAMK2D protein; Calcium dependent protein kinase type II subunit delta; CAMKD; CaM Kinase II Delta Subunit; CAMK2D recombinant protein
Host
Baculovirus
Form/Format
Recombinant CAMK2D protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant CAMK2D protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001212.2) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 57.52kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HTRF assay for CAMK2D activity 1uM STK S1 substrate was incubated with different concentrations of CAMK2D protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 500uM CaCl2,1uM Calmodulin, 1 mM DTT, 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for CAMK2D activity 1uM STK S1 substrate was incubated with different concentrations of CAMK2D protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 500uM CaCl2,1uM Calmodulin, 1 mM DTT, 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant CAMK2D protein 10% SDS-PAGE Comassie staining MW: 57.52kDa Purity: >95%)
SDS-PAGE
(Recombinant CAMK2D protein 10% SDS-PAGE Comassie staining MW: 57.52kDa Purity: >95%)
Related Product Information for CAMK2D recombinant protein
CAMK2D (Calcium/calmodulin-dependent protein kinase type II subunit delta) is a Calcium/calmodulindependent protein kinase involved in the regulation of Ca2+ homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca2+ influx into the myocyte, Ca2+ release from the sarcoplasmic reticulum (SR), SR Ca2+ uptake and Na+ and K+ channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca2+ release via direct phosphorylation of RYR2 Ca2+ channel on ‘Ser-2808’. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program.
Product Categories/Family for CAMK2D recombinant protein
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Product Notes
The CAMK2D (Catalog #AAA60415) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CAMK2D, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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