Standard Curve (Sample)
Canine Osteocalcin (OC) ELISA Kit | OC elisa kit
Canine Osteocalcin (OC) ELISA Kit
Gene Names
BGLAP; OC; BGP; OCN
Reactivity
Canine
Synonyms
Osteocalcin (OC); N/A; Canine Osteocalcin (OC) ELISA Kit; BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein; OC elisa kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC).
No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed.
No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed.
Sequence Length
100
Samples
Serum, plasma and other biological fluids
Assay Type
Competitive
Detection Range
617.28-50000pg/mL
Sensitivity
Typically less than 244.5pg/mL.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteocalcin (OC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards;
2. Add 50uL standard or sample to each well.
And then add 50uL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37 degree C;
3. Aspirate and wash 3 times;
4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
5. Aspirate and wash 5 times;
6. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
7. Add 50uL Stop Solution. Read at 450 nm immediately.
2. Add 50uL standard or sample to each well.
And then add 50uL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37 degree C;
3. Aspirate and wash 3 times;
4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
5. Aspirate and wash 5 times;
6. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
7. Add 50uL Stop Solution. Read at 450 nm immediately.
Preparation and Storage
For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.
For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for OC elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Osteocalcin (OC) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Osteocalcin (OC) and unlabeled Osteocalcin (OC) (Standards or samples) with the pre-coated antibody specific to Osteocalcin (OC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Osteocalcin (OC) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Osteocalcin (OC) in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,963 Da
NCBI Official Full Name
osteocalcin preproprotein
NCBI Official Synonym Full Names
bone gamma-carboxyglutamate (gla) protein
NCBI Official Symbol
BGLAP
NCBI Official Synonym Symbols
OC; BGP; OCN
NCBI Protein Information
osteocalcin
UniProt Protein Name
Osteocalcin
UniProt Gene Name
BGLAP
UniProt Synonym Gene Names
BGP
UniProt Entry Name
OSTCN_HUMAN
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Product Notes
The Canine OC bglap (Catalog #AAA137500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA137500 ELISA Kit recognizes Canine OC. It is sometimes possible for the material contained within the vial of "Osteocalcin (OC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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