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Standard Curve (Sample)

Canine TSH ELISA Kit | TSH elisa kit

TSH (Canine) ELISA

Reactivity
Canine
Synonyms
TSH; TSH (Canine) ELISA; TSH elisa kit
Ordering
For Research Use Only!
Reactivity
Canine
Specificity
The specificity is defined as the probability of the assay of scoring negative in the absence of the spe-cific analyte. (Detected by method comparison with Mikrogen ELISA, with three lots of our ELISA. 77 samples, therefrom 57 negative samples are assayed with ELISA lot 1-3.) It is 100% (for all three lots).
Sequence Length
1377
Assay Type
Quantitative Sandwich
Samples
Serum and EDTA-plasma
Sensitivity
0.00002 ng/ml
Intra-assay Precision
The intra-assay (within-run) precision of the AAA Biotech' Treponema pallidum IgM ELISA was deter-mined by 20 x measurements of 12 serum samples covering the whole measuring range
Inter-assay Precision
The inter-assay variation of the AAA Biotech pallidum IgM ELISA was determined with 3 samples with 2 production kits in 10 independent runs with 2 replicates per run.
Preparation and Storage
2 - 8 degree C

Standard Curve (Sample)

Standard Curve (Sample)
Related Product Information for TSH elisa kit
Principle of the Assay: The test kit is a solid phase enzyme immunometric assay (ELISA) in the microplate format, designed for the quantitative measurement of canine TSH. The microplate is coated with an antibody specific for canine TSH. Calibrators and samples are pipetted into the antibody coated microplate. Afterwards, a horseradish peroxidase-labeled antibody is added. During a two hour incubation sandwich complexes consisting of the two antibodies and the canine TSH is formed. Non-reactive components are removed by a washing step. A chromogenic substrate, TMB (3,3',5,5'-Tetra-Methyl-Benzidine), is added to all wells. During a 30 minutes incubation, the substrate is converted to a colored end product (blue) by the bound enzyme. Enzyme reaction is stopped by dispensing hydrochloric acid as stop solution (change from blue to yellow). The color intensity is direct proportional to the concentration of canine TSH present in the sample. The optical density of the color solution is measured with a microplate reader at 450 nm.

Intended Uses: TSH canine ELISA is an enzyme immunoassay for the quantitative measurement of canine TSH (thyrotropin) in serum and EDTA-plasma. For manual processing! The usage of laboratory automats is the user's sole responsibility. The kit is intended for single use only.
Product Categories/Family for TSH elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
148,227 Da
NCBI Official Full Name
Tsh
UniProt Protein Name
Temperature-sensitive hemagglutinin tsh autotransporter
UniProt Gene Name
tsh
UniProt Entry Name
TSH_ECOLX

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Product Notes

The Canine TSH tsh (Catalog #AAA14212) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA14212 ELISA Kit recognizes Canine TSH. It is sometimes possible for the material contained within the vial of "TSH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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