Atypical chemokine receptor 2 (ACKR2) Recombinant Protein | ACKR2 recombinant protein

Recombinant Human Atypical chemokine receptor 2 (ACKR2)(Active)

Cat. Num. AAA279386

• Gene Names: CCBP2; D6; hD6; CCR9; CCR10; CMKBR9
• Purity: Greater than 85% as determined by SDS-PAGE.
• Synonyms: Atypical chemokine receptor 2 (ACKR2); N/A; Recombinant Human Atypical chemokine receptor 2 (ACKR2)(Active); C-C chemokine receptor D6Chemokine receptor CCR-10Chemokine receptor CCR-9Chemokine-binding protein 2Chemokine-binding protein D6CCBP2, CCR10, CMKBR9, D6; ACKR2 recombinant protein

• Host: E coli (in vitro)
• Purity/Purification: Greater than 85% as determined by SDS-PAGE.
• Form/Format: Liquid or Lyophilized powder. If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Sequence: MAATASPQPLATEDADSENSSFYYYDYLDEVAFMLCRKDAVVSFGKVFLPVFYSLIFVLGLSGNLLLLMVLLRYVPRRRMVEIYLLNLAISNLLFLVTLPFWGISVAWHWVFGSFLCKMVSTLYTINFYSGIFFISCMSLDKYLEIVHAQPYHRLRTRAKSLLLATIVWAVSLAVSIPDMVFVQTHENPKGVWNCHADFGGHGTIWKLFLRFQQNLLGFLLPLLAMIFFYSRIGCVLVRLRPAGQGRALKIAAALVVAFFVLWFPYNLTLFLHTLLDLQVFGNCEVSQHLDYALQVTESIAFLHCCFSPILYAFSSHRFRQYLKAFLAAVLGWHLAPGTAQASLSSCSESSILTAQEEMTGMNDLGERQSENYPNKEDVGNKSA
• Sequence Length: 1-384aa; Full Length

• Species: Homo sapiens (Human)
• Tag: N-terminal 10xHis-tagged
• Transmembrane Domain: 7TM
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized ACKR2 at 1ug/ml can bind human CCL2, the EC50 of human CCL2 protein is 23.52-30.99ug/ml.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.; Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

SDS-PAGE

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

Bioactivity

Related Product Information for ACKR2 recombinant protein

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs.

Product Categories/Family for ACKR2 recombinant protein

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 13929467
• NCBI GeneID: 1238
• NCBI Accession #: NP_001287.2
• NCBI GenBank Nucleotide #: NM_001296.4
• UniProt Accession #: O00590; O00537; Q53YA1; Q86UN9; Q96A02; B2R8Y8
• Molecular Weight: 43,443 Da
• NCBI Official Full Name: chemokine-binding protein 2
• NCBI Official Synonym Full Names: chemokine binding protein 2
• NCBI Official Symbol: CCBP2
• NCBI Official Synonym Symbols: D6; hD6; CCR9; CCR10; CMKBR9
• NCBI Protein Information: chemokine-binding protein 2; chemokine receptor D6; chemokine receptor CCR-9; C-C chemokine receptor D6; chemokine receptor CCR-10; chemokine (C-C) receptor 9; chemokine-binding protein D6; chemokine (C-C motif) receptor 9; CC-chemokine-binding receptor J
• UniProt Protein Name: Chemokine-binding protein 2
• UniProt Gene Name: CCBP2
• UniProt Synonym Gene Names: CCR10; CMKBR9
• UniProt Entry Name: CCBP2_HUMAN