Standard Curve (Sample)
(Fig.1. Human Cation-dependent mannose-6-phosphate receptor (M6PR) Standard Curve.)
Human Cation-Dependent Mannose-6-Phosphate Receptor (M6PR) ELISA Kit | CD-MPR elisa kit
Human Cation-Dependent Mannose-6-Phosphate Receptor (M6PR) ELISA Kit
Gene Names
M6PR; SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
Reactivity
Human
Synonyms
Cation-Dependent Mannose-6-Phosphate Receptor (M6PR); N/A; Human Cation-Dependent Mannose-6-Phosphate Receptor (M6PR) ELISA Kit; M6PR; FLJ32994; MPR46; SMPR; 46-kDa mannose 6-phosphate receptor; Mr 46; 000 Man6PR; cation-dependent mannose-6-phosphate receptor; CD-MPR elisa kit
Reactivity
Human
Specificity
Human Cation-dependent mannose-6-phosphate receptor (M6PR) ELISA Kit has high sensitivity and excellent specificity for detection of Human M6PR. No significant cross-reactivity or interference between Human M6PR and analogues was observed.
Sequence Length
277
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Cation-dependent mannose-6-phosphate receptor (M6PR) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Cation-dependent mannose-6-phosphate receptor (M6PR) Standard Curve.)
Related Product Information for CD-MPR elisa kit
Description: This Human Cation-dependent mannose-6-phosphate receptor (M6PR) ELISA Kit employs a two-site sandwich ELISA to quantitate M6PR in samples. An antibody specific for M6PR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any M6PR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for M6PR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of M6PR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: M6PR is a receptor for mannose-6-phosphate groups on lysosomal enzymes. The receptor forms a homodimer or homotetramer for intracellular targeting of lysosomal enzymes and export of newly synthesized lysosomal enzymes into the cell secretions. The receptor is an integral membrane protein which localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane. Two distinct mannose 6-phosphate receptors have been identified: one is cation-independent and has an apparent molecular weight of 215,000. The second, the cation-dependent mannose 6-phosphate receptor, or small mannose 6-phosphate receptor, has an apparent molecular weight of only 46,000 and requires divalent cations for high-affinity binding with its ligand.
Background: M6PR is a receptor for mannose-6-phosphate groups on lysosomal enzymes. The receptor forms a homodimer or homotetramer for intracellular targeting of lysosomal enzymes and export of newly synthesized lysosomal enzymes into the cell secretions. The receptor is an integral membrane protein which localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane. Two distinct mannose 6-phosphate receptors have been identified: one is cation-independent and has an apparent molecular weight of 215,000. The second, the cation-dependent mannose 6-phosphate receptor, or small mannose 6-phosphate receptor, has an apparent molecular weight of only 46,000 and requires divalent cations for high-affinity binding with its ligand.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cation-dependent mannose-6-phosphate receptor isoform 1
NCBI Official Synonym Full Names
mannose-6-phosphate receptor, cation dependent
NCBI Official Symbol
M6PR
NCBI Official Synonym Symbols
SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
NCBI Protein Information
cation-dependent mannose-6-phosphate receptor
UniProt Protein Name
Cation-dependent mannose-6-phosphate receptor
UniProt Gene Name
M6PR
UniProt Synonym Gene Names
MPR46; MPRD; CD Man-6-P receptor; CD-MPR; MPR 46
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Product Notes
The Human CD-MPR m6pr (Catalog #AAA331906) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331906 ELISA Kit recognizes Human CD-MPR. It is sometimes possible for the material contained within the vial of "Cation-Dependent Mannose-6-Phosphate Receptor (M6PR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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