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product-image-AAA190079_SC15.jpg Standard Curve (Sample)

Mouse Integrin Alpha X ELISA Kit | CD11c elisa kit

Mouse Integrin Alpha X (CD11c) (Sandwich ELISA) ELISA Kit

Gene Names
Itgax; Cr4; N418; Cd11c; AI449405
Reactivity
Mouse
Synonyms
Integrin Alpha X; N/A; Mouse Integrin Alpha X (CD11c) (Sandwich ELISA) ELISA Kit; ITGaX; CD11-C; ITG-AX; ITGA-X; Leu M5; Complement Component 3 Receptor 4 Subunit; CD11 antigen-like family member C; Leukocyte adhesion glycoprotein p150,95 alpha chain; ELISA Kit for Integrin Alpha X (CD11c); CD11c elisa kit
Ordering
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of CD11c. No significant cross-reactivity or interference between CD11c and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Mouse tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.058ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD11c were tested 20 times on one plate, respectively. Intra-Assay:
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD11c were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year

Standard Curve (Sample)

product-image-AAA190079_SC15.jpg Standard Curve (Sample)
Related Product Information for CD11c elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CD11c. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CD11c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD11c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD11c in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD11c in mouse tissue homogenates, cell lysates and other biological fluids.
Product Categories/Family for CD11c elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
129,151 Da
NCBI Official Full Name
integrin alpha-X
NCBI Official Synonym Full Names
integrin alpha X
NCBI Official Symbol
Itgax
NCBI Official Synonym Symbols
Cr4; N418; Cd11c; AI449405
NCBI Protein Information
integrin alpha-X; CD11 antigen-like family member C; CD11C (p150) alpha polypeptide; complement receptor 4; integrin aX; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95
UniProt Protein Name
Integrin alpha-X
UniProt Gene Name
Itgax
UniProt Entry Name
ITAX_MOUSE

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Product Notes

The Mouse CD11c itgax (Catalog #AAA190079) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA190079 ELISA Kit recognizes Mouse CD11c. It is sometimes possible for the material contained within the vial of "Integrin Alpha X, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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