ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Human CD19 (M75V,R76S,F85S) Protein, hFc Tag (AAA129497) can bind Anti-CD19(FMC63 biosimilar) mAb in a linear range of 3.20–400 ng/mL.)
CD19 recombinant protein
Human CD19 (M75V,R76S,F85S) Protein, hFc Tag
Gene Names
CD19; B4; CVID3
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
CD19; N/A; Human CD19 (M75V,R76S,F85S) Protein, hFc Tag; B4; CVID3; Recombinant human CD19 (M75V; R76S; F85S) Protein with C-terminal human Fc tag; CD19 recombinant protein
Host
HEK293
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Sequence
CD19 (M75V,R76S,F85S)(Pro20-Lys291) hFc (Glu99-Ala330)
Tag
C-Human Fc
Preparation and Storage
Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Human CD19 (M75V,R76S,F85S) Protein, hFc Tag (AAA129497) can bind Anti-CD19(FMC63 biosimilar) mAb in a linear range of 3.20–400 ng/mL.)
ELISA
(Figure 2. ELISA plate pre-coated by 2 ug/mL (100 uL/well) Human CD19 (M75V,R76S,F85S) Protein, hFc Tag (AAA129497) can bind Anti-CD19(FMC63 biosimilar) mAb in a linear range of 3.20–400 ng/mL.)
SDS-PAGE
(Figure 1. Human CD19(M75V,R76S,F85S) Protein, hFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human CD19(M75V,R76S,F85S) Protein, hFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for CD19 recombinant protein
This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]
Product Categories/Family for CD19 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,128 Da
NCBI Official Full Name
B-lymphocyte antigen CD19 isoform 1
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
CD19
NCBI Official Synonym Symbols
B4; CVID3
NCBI Protein Information
B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Gene Name
CD19
UniProt Entry Name
CD19_HUMAN
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Product Notes
The CD19 cd19 (Catalog #AAA129497) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: CD19 (M75V,R76S,F85S)(Pro 20-Lys291) hFc (Glu99-Ala 330). It is sometimes possible for the material contained within the vial of "CD19, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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