SDS-PAGE
(Fig.SDS-PAGE analysis of Human CD33/Siglec-3 protein.)
CD33/Siglec-3 Recombinant Protein | CD33 recombinant protein
Human CD33/Siglec-3 protein, His tag (Animal-Free)
Purity
>95% as determined by SDS-PAGE
Synonyms
CD33/Siglec-3; N/A; Human CD33/Siglec-3 protein, His tag (Animal-Free); CD33 Molecule; SIGLEC3; Sialic Acid-Binding Ig-Like Lectin 3; Myeloid Cell Surface Antigen CD33; CD33 Antigen (Gp67); SIGLEC-3; Gp67; Sialic Acid Binding Ig-Like Lectin 3; CD33 Molecule Transcript 3; CD33 Antigen; CD33 recombinant protein
Host
HEK 293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
0.22um filtered protein solution is in PBS, pH7.4.
Sequence Positions
Amino acid sequence derived from human CD33 protein (AAA51948, Met1-His259) was expressed with a polyhistidine tag at the C-terminus.
Endotoxin
<0.1 EU per ug of the protein as determined by the LAL method.
Protein Construction
Amino acid sequence derived from human CD33 protein (AAA51948, Met1-His259) was expressed with a polyhistidine tag at the C-terminus.
Protein Length
The recombinant human CD33/Siglec-3 protein consists of 248 amino acids and predicts a molecular mass of 27.3 kD.The apparent molecular mass of the human CD33 is approximately 40-55kDa in SDS-PAGE under reducing conditions due to glycosylation.
Usage Notes
It is recommended to reconstitute the lyophilized human CD33/Siglec-3 protein using the buffer we provided not less than 100ug/mL, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Lyophilized humanCD33/Siglec-3 protein product should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
SDS-PAGE
(Fig.SDS-PAGE analysis of Human CD33/Siglec-3 protein.)
SDS-PAGE
(Fig.SDS-PAGE analysis of Human CD33/Siglec-3 protein.)
Related Product Information for CD33 recombinant protein
Description: Recombinant Human CD33/Siglec-3 protein expressed in HEK293 Cells.
Background: The transmembrane protein CD33 is a sialic acid-binding immunoglobulin-like lectin that mediate the cell-cell interaction and inhibiting normal functions of immune cells. CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. It may constitute a control mechanism of the innate immune system. In the brain, CD33 is mainly expressed on microglial cells.
Background: The transmembrane protein CD33 is a sialic acid-binding immunoglobulin-like lectin that mediate the cell-cell interaction and inhibiting normal functions of immune cells. CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. It may constitute a control mechanism of the innate immune system. In the brain, CD33 is mainly expressed on microglial cells.
Product Categories/Family for CD33 recombinant protein
NCBI and Uniprot Product Information
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Product Notes
The CD33 cd33 (Catalog #AAA332463) is a Recombinant Protein produced from HEK 293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Amino acid sequence derived from human CD33 protein (AAA51948, Met1-His259) was expressed with a polyhistidine tag at the C-terminus. It is sometimes possible for the material contained within the vial of "CD33/Siglec-3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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