CD40 Ligand/TNFSF5 Recombinant Protein | CD40 recombinant protein

CD40 Ligand/TNFSF5 Protein, Mouse, Recombinant (ECD, His Tag)

Cat. Num. AAA259249

• Gene Names: Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
• Purity: >= 95% as determined by SDS-PAGE.
• Synonyms: CD40 Ligand/TNFSF5; N/A; CD40 Ligand/TNFSF5 Protein, Mouse, Recombinant (ECD, His Tag); CD40 ligand; CD154 Protein, Mouse; CD40-L Protein, Mouse; Cd40l Protein, Mouse; gp39 Protein, Mouse; HIGM1 Protein, Mouse; IGM Protein, Mouse; IMD3 Protein, Mouse; Ly-62 Protein, Mouse; Ly62 Protein, Mouse; T-BAM Protein, Mouse; Tnfsf5 Protein, Mouse; TRAP Protein, Mouse; CD40 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >= 95% as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4.; Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

• Species: Mouse
• Predicted N Terminal: His
• Endotoxin: <1.0 EU per ug protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the Mouse CD40LG (P27548)(Gly115-Leu260) was expressed, with a polyhistidine tag at the N-terminus.
• Activity: Immobilized Recombinant Mouse CD40/TNFRSF5 Protein (Fc Tag)(Cat:50324-M02H) at 2ug/mL (100uL/well) can bind Recombinant Mouse CD40 Ligand Protein (ECD, His Tag)(Cat:50327-M07H), the EC50 is 14-36 ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Application Data

(Immobilized Recombinant Mouse CD40 / TNFRSF5 Protein (Fc Tag) (Cat:50324-M02H) at 2ug/mL (100uL/well) can bind Recombinant Mouse CD40 Ligand Protein (ECD, His Tag) (Cat:AAA259249), the EC50 is 14-36 ng/mL.)

Application Data

Related Product Information for CD40 recombinant protein

The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-gamma from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Grewal IS, et al. (1998) CD40 and CD154 in cell-mediated immunity. Annual Review of Immunology. 16: 111-35.

NCBI and Uniprot Product Information

• NCBI GI #: 15011846
• NCBI GeneID: 21947
• NCBI Accession #: NP_035746.2
• NCBI GenBank Nucleotide #: NM_011616.2
• UniProt Accession #: P27548
• Molecular Weight: 29,370 Da
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: Cd40lg
• NCBI Official Synonym Symbols: IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
• NCBI Protein Information: CD40 ligand
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: Cd40lg
• UniProt Synonym Gene Names: Cd40l; Tnfsf5; CD40-L; TRAP
• UniProt Entry Name: CD40L_MOUSE