CD40 Ligand/TNFSF5 Active Protein | CD40 active protein

Recombinant human CD40 Ligand/TNFSF5 protein

Cat. Num. AAA48396

• Gene Names: CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: CD40 Ligand/TNFSF5; N/A; Recombinant human CD40 Ligand/TNFSF5 protein; CD40 ligand; CD154; CD40L; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP; CD40 active protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl
• Concentration: 0.5mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 113-261aa
• Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LCLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

• Applicable Applications for CD40 active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Bioactivity: Measured by its binding ability in a functional ELISA with Human CD40/TNFRSF5
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human CD40 Ligand/TNFSF5 is coated at 5 ug/ml (100 ul/well) can bind Human CD40/TNFRSF5 (CAT# ATGP4007) in a Functional ELISA assay.)

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stainLane 1 : reducing conditionLane 2 : non-reducing condition)

Related Product Information for CD40 active protein

CD40 ligand, also known as CD40LG, is a member of the TNF superfamily and is expressed on activated T cells. CD40LG is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. Recombinant human CD40LG protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CD40 active protein

Immunology; Proteins

References

Gordon J., et al. (1995) Blood Rev. 9:53-56. Cheng G., et al. (1995) Science. 267:1494-1498.

NCBI and Uniprot Product Information

• NCBI GI #: 4557433
• NCBI GeneID: 959
• NCBI Accession #: NP_000065
• NCBI GenBank Nucleotide #: NM_000074.2
• UniProt Accession #: P29965
• Molecular Weight: 18.3 kDa (169aa), confirmed by MALDI-TOF
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: CD40LG
• NCBI Official Synonym Symbols: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• NCBI Protein Information: CD40 ligand
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: CD40LG
• UniProt Synonym Gene Names: CD40L; TNFSF5; TRAP; CD40-L; TRAP
• UniProt Entry Name: CD40L_HUMAN