SDS-PAGE
CDK8 recombinant protein
CDK8, Unactive recombinant protein
Gene Names
CDK8; K35
Applications
Western Blot
Purity
The purity was determined to be >80% by densitometry.
Synonyms
CDK8; N/A; CDK8, Unactive recombinant protein; K35; MGC126074; MGC126075; Unacitve; CDK8 recombinant protein
Host
Sf9 insect cells
Purity/Purification
The purity was determined to be >80% by densitometry.
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Concentration
0.2ug/ul (varies by lot)
Sequence Length
1464
Applicable Applications for CDK8 recombinant protein
WB (Western Blot), Kinase Assay
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships with Dry Ice.
Shipping: Ships with Dry Ice.
SDS-PAGE
SDS-PAGE
Related Product Information for CDK8 recombinant protein
Recombinant full-length human CDK8 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (1). CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for beta-catenin-driven transformation and for expression of several beta-catenin transcriptional targets (2).
Scientific Background: CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (1). CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for beta-catenin-driven transformation and for expression of several beta-catenin transcriptional targets (2).
Product Categories/Family for CDK8 recombinant protein
References
1.Akoulitchev S, et al: TFIIH is negatively regulated by cdk8-containing mediator complexes. Nature 407: 102-106, 2000.
2.Firestein R, et al: CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature 455: 547-551, 2008.
2.Firestein R, et al: CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature 455: 547-551, 2008.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~84 kDa
NCBI Official Full Name
Homo sapiens cyclin-dependent kinase 8, mRNA
NCBI Official Synonym Full Names
cyclin-dependent kinase 8
NCBI Official Symbol
CDK8
NCBI Official Synonym Symbols
K35
NCBI Protein Information
cyclin-dependent kinase 8; protein kinase K35; CDK8 protein kinase; mediator complex subunit CDK8; cell division protein kinase 8; mediator of RNA polymerase II transcription subunit CDK8
UniProt Protein Name
Cyclin-dependent kinase 8
UniProt Gene Name
CDK8
UniProt Entry Name
CDK8_HUMAN
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Product Notes
The CDK8 cdk8 (Catalog #AAA72888) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CDK8 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the CDK8 cdk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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