Application Data
(ADP-Glo assay for CDK9/CyclinT1 Complex activity 0.2 ug/ul PDKtide peptide substrate was incubated with different concentrations of CDK9/CyclinT1 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
CDK9/CyclinT1 recombinant protein
Recombinant CDK9/CyclinT1 protein complex
Synonyms
CDK9/CyclinT1; N/A; Recombinant CDK9/CyclinT1 protein complex; Cyclin Dependent Kinase 9; TAK; PITALRE; CDC2L4; C-2k; Cell Division Cycle 2-Like Protein Kinase 4; CTK1; CDK9/CyclinT1 recombinant protein
Host
Baculovirus
Form/Format
Recombinant CDK9/CyclinT1 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant CDK9/CyclinT1 Complex that includes full length human CDK9 protein (accession number NP_001252.1) without tag and full-length human Cyclin-T1 protein (accession number NP_001231.2) with an N-terminal DYKDDDDK tag was expressed in Hi5 cells. The molecular weights of CDK9 and CyclinT1 are 42.76kDa and 81.95kDa, respectively.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(ADP-Glo assay for CDK9/CyclinT1 Complex activity 0.2 ug/ul PDKtide peptide substrate was incubated with different concentrations of CDK9/CyclinT1 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
Application Data
(ADP-Glo assay for CDK9/CyclinT1 Complex activity 0.2 ug/ul PDKtide peptide substrate was incubated with different concentrations of CDK9/CyclinT1 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
SDS-PAGE
(Recombinant CDK9/CyclinT1 complex 10% SDS-PAGE Coomassie staining MW: CDK9: 42.76kDa, Cyclin-T1:81.95kDa Purity: >=90%)
SDS-PAGE
(Recombinant CDK9/CyclinT1 complex 10% SDS-PAGE Coomassie staining MW: CDK9: 42.76kDa, Cyclin-T1:81.95kDa Purity: >=90%)
Related Product Information for CDK9/CyclinT1 recombinant protein
CDK9 is a member of the CDC2-like family of kinases. Its cyclin partners are members of the CYCLIN T family (T1, T2a, and T2b) and CYCLIN K. The CDK9/CYCLIN T1 complex is very important in the differentiation programming of several cell types, including muscle cells and neurons, controlling specific differentiation pathways.
Product Categories/Family for CDK9/CyclinT1 recombinant protein
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Product Notes
The CDK9/CyclinT1 (Catalog #AAA60416) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CDK9/CyclinT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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