SDS-PAGE
(The purity of STK9 was determined to be >85% by densitometry, approx MW 84 kDa.)
STK9 recombinant protein
STK9 recombinant protein
Gene Names
CDKL5; ISSX; STK9; EIEE2
Applications
Western Blot
Purity
>85%
Synonyms
STK9; N/A; STK9 recombinant protein; CDKL5; EIEE2; ISSX
Host
Sf9 insect cells
Purity/Purification
>85%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1 ug/ uL (varies by lot)
Sequence Positions
1-498
Sequence Length
3434
Applicable Applications for STK9 recombinant protein
WB (Western Blot)
Source Note
Recombinant human STK9 (CDKL5) (1-498) was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Ships with Dry Ice.
SDS-PAGE
(The purity of STK9 was determined to be >85% by densitometry, approx MW 84 kDa.)
SDS-PAGE
(The purity of STK9 was determined to be >85% by densitometry, approx MW 84 kDa.)
Related Product Information for STK9 recombinant protein
Recombinant human STK9 (CDKL5) (1-498) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics (1). The mutations in the STK9 is a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder (2).
Scientific Background: STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics (1). The mutations in the STK9 is a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder (2).
Product Categories/Family for STK9 recombinant protein
References
1. Ricciardi, S. et.al: CDKL5 influences RNA splicing activity by its association to the nuclear speckle molecular machinery. Hum. Molec. Genet. 18: 4590-4602, 2009.
2. Archer, H. L. et.al: CDKL5 mutations cause infantile spasms, early onset seizures, and severe mental retardation in female patients. J. Med. Genet. 43: 729-734, 2006.
2. Archer, H. L. et.al: CDKL5 mutations cause infantile spasms, early onset seizures, and severe mental retardation in female patients. J. Med. Genet. 43: 729-734, 2006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~84 kDa
NCBI Official Full Name
Homo sapiens cyclin-dependent kinase-like 5 (CDKL5), transcript variant I, mRNA
NCBI Official Synonym Full Names
cyclin-dependent kinase-like 5
NCBI Official Symbol
CDKL5
NCBI Official Synonym Symbols
ISSX; STK9; EIEE2
NCBI Protein Information
cyclin-dependent kinase-like 5; serine/threonine kinase 9; serine/threonine-protein kinase 9; cyclin dependent kinase 5 transcript
UniProt Protein Name
Cyclin-dependent kinase-like 5
UniProt Gene Name
CDKL5
UniProt Synonym Gene Names
STK9
UniProt Entry Name
CDKL5_HUMAN
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Product Notes
The STK9 cdkl5 (Catalog #AAA72912) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-498. AAA Biotech's STK9 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the STK9 cdkl5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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