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product-image-AAA189992_SC15.jpg Standard Curve (Sample) (Typical Standard Curve for MYH10 ELISA (Sandwich))

Mouse Myosin Heavy Chain 10, Non Muscle ELISA Kit | MYH10 elisa kit

Mouse Myosin Heavy Chain 10, Non Muscle (MYH10) (Sandwich ELISA) ELISA Kit

Gene Names
Myh10; Fltn; Myhn2; SMemb; Myhn-2; NMHC-B; NMMHC-B; NMHCII-B; NMHC II-B; NMMHC-IIB; mKIAA3005; NMMHC II-b; 5730504C04Rik; 9330167F11Rik
Reactivity
Mouse
Synonyms
Myosin Heavy Chain 10, Non Muscle; N/A; Mouse Myosin Heavy Chain 10, Non Muscle (MYH10) (Sandwich ELISA) ELISA Kit; NMMHCB; Cellular myosin heavy chain, type B; Myosin heavy chain, non-muscle IIb; ELISA Kit for Myosin Heavy Chain 10, Non Muscle (MYH10); MYH10 elisa kit
Ordering
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 10, Non Muscle (MYH10).
No significant cross-reactivity or interference between Myosin Heavy Chain 10, Non Muscle (MYH10) and analogues was observed. Reactive with Mouse MYH10 / Myosin Heavy Chain 10, Non Muscle
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.057ng/mL
Detection Method
Colormetric
Assay Precision
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Preparation and Storage
4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year

Standard Curve (Sample)

(Typical Standard Curve for MYH10 ELISA (Sandwich))

product-image-AAA189992_SC15.jpg Standard Curve (Sample) (Typical Standard Curve for MYH10 ELISA (Sandwich))
Related Product Information for MYH10 elisa kit
Principle of the Assay||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 10, Non Muscle (MYH10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 10, Non Muscle (MYH10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 10, Non Muscle (MYH10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 10, Non Muscle (MYH10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for MYH10 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
228,996 Da
NCBI Official Full Name
myosin-10
NCBI Official Synonym Full Names
myosin, heavy polypeptide 10, non-muscle
NCBI Official Symbol
Myh10
NCBI Official Synonym Symbols
Fltn; Myhn2; SMemb; Myhn-2; NMHC-B; NMMHC-B; NMHCII-B; NMHC II-B; NMMHC-IIB; mKIAA3005; NMMHC II-b; 5730504C04Rik; 9330167F11Rik
NCBI Protein Information
myosin-10; flectin; myosin IIB; non-muscle myosin heavy chain B; myosin heavy chain 10, non-muscle; non-muscle myosin heavy chain IIb; nonmuscle myosin heavy chain II-B; myosin heavy chain, non-muscle IIb; cellular myosin heavy chain, type B; myosin heavy
UniProt Protein Name
Myosin-10
UniProt Gene Name
Myh10
UniProt Synonym Gene Names
NMMHC-B; NMMHC II-b; NMMHC-IIB
UniProt Entry Name
MYH10_MOUSE

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Product Notes

The Mouse MYH10 myh10 (Catalog #AAA189992) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189992 ELISA Kit recognizes Mouse MYH10. It is sometimes possible for the material contained within the vial of "Myosin Heavy Chain 10, Non Muscle, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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