p19 INK4d Recombinant Protein | CDKN2D recombinant protein

p19 INK4d Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA257441

• Gene Names: CDKN2D; p19; INK4D; p19-INK4D
• Purity: >90 % as determined by SDS-PAGE
• Synonyms: p19 INK4d; N/A; p19 INK4d Protein, Human, Recombinant (GST Tag); Human CDKN2D/p19ink4d Protein (GST Tag); cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4); INK4D Protein, Human; p19 Protein, Human; p19-INK4D Protein, Human; CDKN2D recombinant protein

• Host: E Coli
• Purity/Purification: >90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 400aa
• Sequence: Met10-Leu166

• Species: Human
• Tag: N-GST
• Predicted N Terminal: Met
• Protein Construction: |A DNA sequence encoding the human CDKN2D (P55273) (Met 10Leu 166) was fused with the GST tag at the N-terminus
• Activity: Immobilized human GST-CDKN2D at 10ug/ml (100ul/well) can bind biotinylated human GST-CDK4 (Cat:10732-H09B). The EC50 of biotinylated human GST-CDK4 (Cat:10732-H09B) is 0.52-1.2ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Immobilized human GST-CDKN2D at 10 ug/ml (100 ul/well) can bind biotinylated human GST-CDK4 (Cat: 10732-H09B), The EC50 of biotinylated human GST-CDK4 (Cat: 10732-H09B) is 0.52-1.2 ug/ml.)

Application Data

Related Product Information for CDKN2D recombinant protein

Cyclin-dependent kinase inhibitor 2D(also known as CDKN2D or p19ink4d), a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, negatively regulates the cyclin D-CDK4/6 complexes, which promote G1/S transition by phosphorylating the retinoblastoma tumor-suppressor gene product. It is clearly shown that DNA repair is the main target of p19ink4d effect and that diminished apoptosis is a downstream event. Experiments has uncovered a role of p19INK4d as a regulator of DNA-damage-induced apoptosis and suggest that it protects cells from undergoing apoptosis by allowing a more efficient DNA repair. It has been demonstrated that p19INK4d expression enhances cell survival under genotoxic conditions. Previous work has shown that inactivation of the cyclin-dependent kinase inhibitor (CKI) p19(Ink4d) leads to progressive hearing loss attributable to inappropriate DNA replication and subsequent apoptosis of hair cells. It may also involved in male reproductive function including testicular atrophy, alteration in serum follicle stimulating hormone, qualitative increase in germ cell apoptosis, and delayed kinetics of meiotic prophase markers.

References

Buchold GM, et al. (2007) Mice lacking cyclin-dependent kinase inhibitor p19Ink4d show strain-specific effects on male reproduction. Mol Reprod Dev. 74 (8): 1008-20. Laine H, et al. (2007) p19(Ink4d) and p21(Cip1) collaborate to maintain the postmitotic state of auditory hair cells, their codeletion leading to DNA damage and p53-mediated apoptosis. J Neurosci. 27 (6): 1434-44.Ceruti JM, et al. (2005) Induction of p19INK4d in response to ultraviolet light improves DNA repair and confers resistance to apoptosis in neuroblastoma cells. Oncogene. 24 (25): 4065-80.

NCBI and Uniprot Product Information

• NCBI GI #: 4502753
• NCBI GeneID: 1032
• NCBI Accession #: NP_001791.1
• NCBI GenBank Nucleotide #: NM_001800.3
• UniProt Accession #: P55273; Q13102; Q6FGE9
• Molecular Weight: 17,700 Da
• NCBI Official Full Name: cyclin-dependent kinase 4 inhibitor D
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
• NCBI Official Symbol: CDKN2D
• NCBI Official Synonym Symbols: p19; INK4D; p19-INK4D
• NCBI Protein Information: cyclin-dependent kinase 4 inhibitor D; CDK inhibitor p19INK4d; inhibitor of cyclin-dependent kinase 4d; cyclin-dependent kinase 4 inhibitor D p19; cell cycle inhibitor, Nur77 associating protein
• UniProt Protein Name: Cyclin-dependent kinase 4 inhibitor D
• UniProt Gene Name: CDKN2D
• UniProt Entry Name: CDN2D_HUMAN