anti-Human, Mouse IL23A / IL-23 p19 Polyclonal Antibody | anti-IL23A antibody

Rabbit Polyclonal to Human IL23A / IL-23 p19

Cat. Num. AAA50714

• Gene Names: IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Peptide Column Affinity Chromatography
• Synonyms: IL23A / IL-23 p19, Antibody; Rabbit Polyclonal to Human IL23A / IL-23 p19; Anti-IL23A / IL-23 p19 Antibody (N-Terminus) IHC-plus; IL23A; IL-23 subunit alpha; IL-23A; Interleukin 23 p19 subunit; Interleukin-23 subunit alpha; IL23P19; Interleukin-23 subunit p19; p19; SGRF; IL-23; IL-23 p19; IL-23-A; IL-23p19; Human IL23A; anti-IL23A antibody

• Host: IL23A/IL-23 P19 antibody was produced in Rabbit.
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: 16 amino acid peptide from near the amino terminus of human IL-23
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-IL23A antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Target Species: IL23A/IL-23 p19
• Immunogen Species: IL23A/IL-23 p19 antibody was raised against Human.
• Antigen Type: Synthetic Peptide
• Usage: IL-23 antibody can be used for the detection of IL-23 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 21 kDa Observed: 23 kDa
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Store at 4°C for up to 3 months. store at -20°C for up to 1 year. Avoid repeated freeze/ thaw cycles.

WB (Western Blot)

(Western blot of IL-23 in mouse pancreas tissue lysate with IL-23 antibody at (A) 1 and (B) 2 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescence of IL-23 in Mouse Pancreas cells with IL-23 antibody at 20 ug/ml.)

IHC (Immunohiostchemistry)

(Anti-IL-23 p19 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-IL-23 p19 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-IL23A antibody

IL23 p19 (IL23A) is a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of the IL23A subunit and the p40 subunit of interleukin 12 (IL12B). Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naïve CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.

Product Categories/Family for anti-IL23A antibody

Family: Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 7706702
• NCBI GeneID: 51561
• NCBI Accession #: NP_057668.1
• NCBI GenBank Nucleotide #: NM_016584.2
• UniProt Accession #: Q9NPF7; Q6NZ80; Q6NZ82; Q9H2A5
• NCBI Official Full Name: interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23, alpha subunit p19
• NCBI Official Symbol: IL23A
• NCBI Official Synonym Symbols: P19; SGRF; IL-23; IL-23A; IL23P19
• NCBI Protein Information: interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor
• UniProt Protein Name: Interleukin-23 subunit alpha
• UniProt Gene Name: IL23A
• UniProt Synonym Gene Names: SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19
• UniProt Entry Name: IL23A_HUMAN