Standard Curve (Sample)
Human CEA ELISA Kit | CEACAM5 elisa kit
Human CEA PicoKine ELISA Kit
Gene Names
CEACAM5; CEA; CD66e
Reactivity
Human
Synonyms
CEA; N/A; Human CEA PicoKine ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen ; Carcinoembryonic antigen related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM 5; CEACAM5; DKFZp781M2392; Meconium antigen 100; CEACAM5 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CEACAM5
Sequence Length
702
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CEACAM5 elisa kit
Background: Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Principle of the Assay: The Picokine™ Human CEACAM5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CEACAM5 with a 96-well strip plate that is pre-coated with antibody specific for CEACAM5. The detection antibody is a biotinylated antibody specific for CEACAM5. The capture antibody is polyclonal antibody from rabbit and the detection antibody is polyclonal antibody from rabbit. The kit contains recombinant Human CEACAM5 with immunogen: Expression system for standard: NS0; Immunogen sequence: K35-A685. The kit is analytically validated with ready-to-use reagents. To measure Human CEACAM5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CEACAM5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CEACAM5 in the sample.
Principle of the Assay: The Picokine™ Human CEACAM5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CEACAM5 with a 96-well strip plate that is pre-coated with antibody specific for CEACAM5. The detection antibody is a biotinylated antibody specific for CEACAM5. The capture antibody is polyclonal antibody from rabbit and the detection antibody is polyclonal antibody from rabbit. The kit contains recombinant Human CEACAM5 with immunogen: Expression system for standard: NS0; Immunogen sequence: K35-A685. The kit is analytically validated with ready-to-use reagents. To measure Human CEACAM5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CEACAM5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CEACAM5 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,724 Da
NCBI Official Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN
Similar Products
Product Notes
The Human CEACAM5 ceacam5 (Catalog #AAA45195) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45195 ELISA Kit recognizes Human CEACAM5. It is sometimes possible for the material contained within the vial of "CEA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.