Standard Curve (Sample)
TNF-alpha cell line
TNF-alpha Leeporter HEK293 Cell Line
Gene Names
TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
Applications
Functional Assay
Synonyms
TNF-alpha; N/A; TNF-alpha Leeporter HEK293 Cell Line; TNF-alpha cell line
Form/Format
Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Sequence Length
233
Applicable Applications for TNF-alpha cell line
FA (Functional Assay)
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Functional validation
A. Response of TNF-alpha Leeporter™ – HEK293 cells to phorbol 12-myristate 13-acetate.
1. Harvest TNF-alpha Leeporter™ – HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 10^4 cells/well.
2. Incubate cells at 37 degree C in a CO2 incubator for overnight.
3. The next day, stimulate cells with different concentrations of phorbol 12-myristate 13-acetate (PMA).
4. Incubate at 37 degree C in a CO2 incubator for 6-16 hours.
5. Add 50 ul of luciferase assay reagent (Cat #MBS668917) per well.
6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.
1. Harvest TNF-alpha Leeporter™ – HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 10^4 cells/well.
2. Incubate cells at 37 degree C in a CO2 incubator for overnight.
3. The next day, stimulate cells with different concentrations of phorbol 12-myristate 13-acetate (PMA).
4. Incubate at 37 degree C in a CO2 incubator for 6-16 hours.
5. Add 50 ul of luciferase assay reagent (Cat #MBS668917) per well.
6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.
Preparation and Storage
Immediately upon receipt, store in liquid nitrogen.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNF-alpha cell line
Principle of the Assay: The TNF-alpha Leeporter™ Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-alpha promoter. Tumor necrosis factor-alpha (TNF-alpha) is one of the major proinflammatory cytokines and can induce systemic inflammation, apoptotic cell death, sepsis and cachexia. Dysregulation of TNF-alpha induction is often involved in various human diseases including inflammatory bowel disease, cancer and Alzheimer's disease. The TNF-alpha induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.
Product Categories/Family for TNF-alpha cell line
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
TNF-alpha
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
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Product Notes
The TNF-alpha tnf (Catalog #AAA78384) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNF-alpha can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay). Researchers should empirically determine the suitability of the TNF-alpha tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF-alpha, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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