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SDS-PAGE

CFD recombinant protein

CFD, 21-253aa, Human, His tag, Baculovirus

Gene Names
CFD; DF; ADN; PFD; ADIPSIN
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
CFD; N/A; CFD, 21-253aa, Human, His tag, Baculovirus; Complement factor D preproprotein; ADIPSIN; ADN; DF; PFD; CFD recombinant protein
Ordering
 
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For Research Use Only!
Host
Baculovirus
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4).
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
21-253aa
Sequence
PPRGRILGGR EAEAHARPYM ASVQLNGAHL CGGVLVAEQW VLSAAHCLED AADGKVQVLL GAHSLSQPEP SKRLYDVLRA VPHPDSQPDT IDHDLLLLQL SEKATLGPAV RPLPWQRVDR DVAPGTLCDV AGWGIVNHAG RRPDSLQHVL LPVLDRATCN RRTHHDGAIT ERLMCAESNR RDSCKGDSGG PLVCGGVLEG VVTSGSRVCG NRKKPGIYTR VASYAAWIDS VLAVEHHHHH H
Sequence Length
253
Applicable Applications for CFD recombinant protein
SDS-PAGE
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for CFD recombinant protein
CFD, also known as Complement factor D preproprotein, is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. There are two antiparallel beta-barrel domains with each barrel containing six beta-strands with the same typology in all enzymes. The major difference in backbone structure between Factor D and the other serine proteases of the chymotrpsin family is in the surface loops connecting the secondary structural elements. Factor D displays different conformations of major catalytic and substrate-binding residues typically found in the chrotrypsin family. Recombinant human CFD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CFD recombinant protein
References
Ronti T. et al. (2006) Clin. Endocrinol. 64 (4):355-65.; Volanakis JE. et al. (1996). Protein Sci. 5 (4):553-64.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26.01kDa (241aa) confirmed by MALDI-TOF
NCBI Official Full Name
complement factor D isoform 1 preproprotein
NCBI Official Synonym Full Names
complement factor D
NCBI Official Symbol
CFD
NCBI Official Synonym Symbols
DF; ADN; PFD; ADIPSIN
NCBI Protein Information
complement factor D
UniProt Protein Name
Complement factor D
UniProt Gene Name
CFD
UniProt Synonym Gene Names
DF; PFD

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Product Notes

The CFD cfd (Catalog #AAA11829) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-253aa with tag His-tag. AAA Biotech's CFD can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CFD cfd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PPRGRILGGR EAEAHARPYM ASVQLNGAHL CGGVLVAEQW VLSAAHCLED AADGKVQVLL GAHSLSQPEP SKRLYDVLRA VPHPDSQPDT IDHDLLLLQL SEKATLGPAV RPLPWQRVDR DVAPGTLCDV AGWGIVNHAG RRPDSLQHVL LPVLDRATCN RRTHHDGAIT ERLMCAESNR RDSCKGDSGG PLVCGGVLEG VVTSGSRVCG NRKKPGIYTR VASYAAWIDS VLAVEHHHHH H. It is sometimes possible for the material contained within the vial of "CFD, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.