Chk2 ELISA Kit | CHEK2 elisa kit

Chk2 (Phospho-Thr68) Phospho Sandwich ELISA Kit

Cat. Num. AAA318620

• Gene Names: CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
• Reactivity: Human, Mouse, Rat
• Synonyms: Chk2; N/A; Chk2 (Phospho-Thr68) Phospho Sandwich ELISA Kit; Cds1; CHEK2; CHK2; CHK2 checkpoint homolog (S. pombe); kinase Chk2; RAD53; CHEK2 elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The Chk2 (Phospho-Thr68) Phospho Sandwich ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can detect Chk2 protein phosphorylation and expression profile in cell lysates. The kit can be used for measuring the relative amounts of phosphorylated Chk2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Chk2 phosphorylation.
• Sequence Length: 586
• Assay Type: Quantitative Sandwich

• Preparation and Storage: Store entire kit at 4 degree C until use. Kit expiration is 3 months from date of shipment.

ELISA

(Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-Chk2 (Phospho-Thr68) Antibody.)

Application Data

(HeLa cells were grown to 90% confluency and were stimulated with PMA 150 ng/mL for 30 minutes. Cells were immediately lysed thereafter and measured for Total Protein Concentration and O.D. 450 nm of Chk2 P-Thr68 versus untreated HeLa lysates.)

Related Product Information for CHEK2 elisa kit

Principle of the Assay: The Phospho Sandwich Chk2 (Phospho-Thr68) ELISA Kit contains the components necessary for semi-quantitative determination of Chk2 (Phospho-Thr68) concentrations within experimental cell lysate samples. This particular immunoassay utilizes the traditional "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope Chk2 (Phospho-Thr68) while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer where data analysis may begin.

Product Categories/Family for CHEK2 elisa kit

Phospho Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 54112407
• NCBI GeneID: 11200
• NCBI Accession #: NP_001005735.1
• NCBI GenBank Nucleotide #: NP_001005735.1
• UniProt Accession #: O96017
• NCBI Official Full Name: serine/threonine-protein kinase Chk2 isoform c
• NCBI Official Synonym Full Names: checkpoint kinase 2
• NCBI Official Symbol: CHEK2
• NCBI Official Synonym Symbols: CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
• NCBI Protein Information: serine/threonine-protein kinase Chk2
• UniProt Protein Name: Serine/threonine-protein kinase Chk2
• UniProt Gene Name: CHEK2
• UniProt Synonym Gene Names: CDS1; CHK2; RAD53; Hucds1; hCds1
• UniProt Entry Name: CHK2_HUMAN