Mouse CHEK2 Monoclonal Antibody | anti-CHEK2 antibody

CHEK2 Mouse Monoclonal Antibody [Clone PCRP-CHEK2-1A4]

Cat. Num. AAA215543

• Gene Names: CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
• Reactivity: Human; Predicted to react with Mouse and Rat.
• Applications: Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CHEK2, Antibody; CHEK2 Mouse Monoclonal Antibody [Clone PCRP-CHEK2-1A4]; Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; CHK2 checkpoint homolog (S. pombe); hCds1; HuCds 1; LFS2; PP1425; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2; anti-CHEK2 antibody

• Host: Mouse
• Reactivity: Human; Predicted to react with Mouse and Rat.
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-CHEK2-1A4
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-CHEK2 antibody: IF (Immunofluorescence), WB (Western Blot), FCM/FACS (Flow Cytometry), IP (Immunoprecipitation)
• Immunogen: Recombinant fragment of human CHEK2 corresponding to protein domain
• Cellular Localization: Nucleus
• Positive Control: HeLa or HCT 116 cells. Human testis, spleen or colon.
• Hu-chromosome Location: 22q12.1
• Unigene: 291363; 505297
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified CHEK2 Mouse Monoclonal Antibody (PCRP-CHEK2-1A4). Confirmation of Purity and Integrity of Antibody.)

Related Product Information for anti-CHEK2 antibody

Recognizes a serine/threonine protein kinase that is a required check-point to mediate cell cycle arrest, activation of DNA repair and apoptosis. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. Also, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 54112407
• NCBI GeneID: 11200
• NCBI Accession #: NP_001005735.1
• NCBI GenBank Nucleotide #: NM_001005735.1
• UniProt Accession #: O96017; Q6QA03; Q6QA04; Q6QA05; Q6QA06; Q6QA07; Q6QA08; Q6QA10; A8K3Y9; B7ZBF3; B7ZBF4; B7ZBF5
• Molecular Weight: 60,915 Da
• NCBI Official Full Name: serine/threonine-protein kinase Chk2 isoform c
• NCBI Official Synonym Full Names: checkpoint kinase 2
• NCBI Official Symbol: CHEK2
• NCBI Official Synonym Symbols: CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
• NCBI Protein Information: serine/threonine-protein kinase Chk2; cds1 homolog; CHK2 checkpoint homolog; checkpoint-like protein CHK2
• UniProt Protein Name: Serine/threonine-protein kinase Chk2
• UniProt Gene Name: CHEK2
• UniProt Synonym Gene Names: CDS1; CHK2; RAD53; Hucds1; hCds1
• UniProt Entry Name: CHK2_HUMAN