CD79a Antibody | anti-CD79A antibody

Anti-Hu CD79a FITC

Cat. Num. AAA129229

• Gene Names: CD79A; IGA; MB-1
• Reactivity: Dog, Chicken, Rabbit, Guinea pig, Horse, Cow, Mouse, Rat, Pig, Non-human primates, Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD79a; N/A; Anti-Hu CD79a FITC; CD79a molecule; BCR alpha; Ig-alpha; MB-1; IGA; anti-CD79A antibody

• Reactivity: Dog, Chicken, Rabbit, Guinea pig, Horse, Cow, Mouse, Rat, Pig, Non-human primates, Human
• Isotype: Mouse IgG1 kappa
• Clone Number: HM47
• Specificity: The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
• Form/Format: FITC; Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 1

• Applicable Applications for anti-CD79A antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD19 positive CD79a positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD79a (HM47) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor intracellular staining pattern of human lymphocytes using anti-human CD79a (HM47) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human CD79a (HM47) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD79A antibody

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

References

*Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol. 2007 Aug;128(2):306-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=bhargava+p+ross+js+azumi+n,*Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/1747162?ordinal,*Rassenti LZ, Kipps TJ: Expression of Ig-beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy-chain allelic exclusion. Blood. 2000 Apr 15;95(8):2725-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/10753858,*Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors. Vet Pathol. 2005 Jul;42(4):437-45., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=fernandez+nj+west+kh,*Torlakovic E, Torlakovic G: B-cell markers in lymphocyte predominance Hodgkin disease. Arch Pathol Lab Med. 2002 Jul;126(7):862-3., URL: http://www.ncbi.nlm.nih.gov/pubmed/12123231,*Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology. Int J Clin Exp Pathol. 2008 Jan 1;1(1):65-74., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hassan+a+perry+a+ning+y,*Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein-Barr virus. J Virol. 2004 Dec;78(24):13891-900., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=netto+g+garcia+jv+cravens+pd,
*Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a)- and Igbeta (CD79b)-mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177(11):7913-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/17114463?ordina,*Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174(3):1245-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/15661879?ordina,*Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173(2):1000-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/15240688?ordina,*Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172(4):2210-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/14764688?ordina,*Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194(11):1583-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/11733573?ordina,

NCBI and Uniprot Product Information

• NCBI GI #: 4502685
• NCBI GeneID: 973
• NCBI Accession #: NP_001774.1
• NCBI GenBank Nucleotide #: NM_001783.3
• UniProt Accession #: P11912; Q53FB8; A0N775
• Molecular Weight: 25,038 Da
• NCBI Official Full Name: B-cell antigen receptor complex-associated protein alpha chain isoform 1
• NCBI Official Synonym Full Names: CD79a molecule, immunoglobulin-associated alpha
• NCBI Official Symbol: CD79A
• NCBI Official Synonym Symbols: IGA; MB-1
• NCBI Protein Information: B-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; surface IgM-associated protein; CD79a antigen (immunoglobulin-associated alpha); membrane-bound immunoglobulin-associated protein
• UniProt Protein Name: B-cell antigen receptor complex-associated protein alpha chain
• UniProt Gene Name: CD79A
• UniProt Synonym Gene Names: IGA; MB1
• UniProt Entry Name: CD79A_HUMAN