Klotho Active Protein | KL active protein
Klotho protein
Purity
> 98% pure
Synonyms
Klotho; N/A; Klotho protein; aKlotho protein; KLA protein; KL active protein
Host
CHO cells
Purity/Purification
> 98% pure
Form/Format
Lyophilized from 15mM Sodium Phosphate pH 7.3 0.2 micron filtered. Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Concentration
20 ug/vial; lyophilized (varies by lot)
Sequence
EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH.
Protein Type
Recombinant
Biological Significance
Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon,. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid.
Bioactivity
Determined by the dose-dependent stimulation of the proliferation of Mouse NIH-3T3 cells. Recombinant human Klotho is effective in a concentration range of 0.5-2.0 ug/ml.
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Species
Human
Additional Remarks
Effective at a concentration of 0.5-2.0 ug/ml
Preparation and Storage
Ships ambient of refrigerated. Upon receipt store at 4 degree C short term, -20 degree C long term. After reconstituted, the solution can be stored at 4 degree C for up to 1 week. Working aliquots stored with a carrier protein are stable for at least 3 months at -20 degree C. Avoid repeated freeze-thaw cycles.
Related Product Information for KL active protein
Purified recombinant Human Klotho protein
Product Categories/Family for KL active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,135 Da
NCBI Official Full Name
klotho
NCBI Official Synonym Full Names
klotho
NCBI Official Symbol
KL
NCBI Protein Information
klotho
UniProt Protein Name
Klotho
UniProt Gene Name
KL
UniProt Entry Name
KLOT_HUMAN
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Product Notes
The KL kl (Catalog #AAA75575) is an Active Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH. It is sometimes possible for the material contained within the vial of "Klotho, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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