Collagen Type IV Alpha 3 Recombinant Protein | COL4A3 recombinant protein
Recombinant Human Collagen Type IV Alpha 3, Biotinylated
Purity
Greater than 87% as determined by Capillary Electrophoresis.
Synonyms
Collagen Type IV Alpha 3; N/A; Recombinant Human Collagen Type IV Alpha 3, Biotinylated; Collagen Type IV Alpha 3 Human Recombinant, Biotinylated; Collagen alpha-3(IV) chain; Goodpasture antigen; COL4A3; Glomerular Basal Membrane; GBM; COL4A3 Human, Biotin; COL4A3 recombinant protein
Host
Sf9 insect cells.
Purity/Purification
Greater than 87% as determined by Capillary Electrophoresis.
Form/Format
Sterile filtered clear solution.
COL4A3 Human biotin (0.9 mg/mL) contains 20mM HEPES, pH 7.5, 0.02% SDS and 0.01mM EDTA.
COL4A3 Human biotin (0.9 mg/mL) contains 20mM HEPES, pH 7.5, 0.02% SDS and 0.01mM EDTA.
Sequence Length
199
Immunological Functions
1. Binds IgG type human auto antibodies.
2. Functional Streptavidin based ELISA test (analysis of positive/negative samples.)
2. Functional Streptavidin based ELISA test (analysis of positive/negative samples.)
Note
It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Avoid multiple freeze-thaw cycles.
Related Product Information for COL4A3 recombinant protein
Description: Human alpha3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Calculated Molecular weight: 42kDa. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV alpha3 chain fused to a 6-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Introductions: Type IV collagen is a major structural component of basement membranes. It is a multimeric protein composed of 3 alpha subunits, which are encoded by 6 different genes, alpha 1 through alpha 6. Each of these alpha subunits can form a triple helix structure with 2 other subunits to form type IV collagen. The Goodpasture syndrome is a condition in which autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are restricted basically to the non-collagenous C-terminal domain of the protein. There are numerous alternative transcripts that appear to be unique to the human COL4A3 and alternative splicing is limited to the six exons that encode this C-terminal domain. COL4A3 is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to the Alport syndrome are also situated within the exons that encode this C-terminal region. COL4A3 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Several exons of COL4A3 are interspersed with exons of an uncharacterized gene which is on the opposite strand.
Introductions: Type IV collagen is a major structural component of basement membranes. It is a multimeric protein composed of 3 alpha subunits, which are encoded by 6 different genes, alpha 1 through alpha 6. Each of these alpha subunits can form a triple helix structure with 2 other subunits to form type IV collagen. The Goodpasture syndrome is a condition in which autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are restricted basically to the non-collagenous C-terminal domain of the protein. There are numerous alternative transcripts that appear to be unique to the human COL4A3 and alternative splicing is limited to the six exons that encode this C-terminal domain. COL4A3 is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to the Alport syndrome are also situated within the exons that encode this C-terminal region. COL4A3 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Several exons of COL4A3 are interspersed with exons of an uncharacterized gene which is on the opposite strand.
Product Categories/Family for COL4A3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
158,336 Da
NCBI Official Full Name
collagen type IV alpha 3, partial
NCBI Official Synonym Full Names
collagen type IV alpha 3 chain
NCBI Official Symbol
COL4A3
NCBI Protein Information
collagen alpha-3(IV) chain
UniProt Protein Name
Collagen alpha-3(IV) chain
UniProt Gene Name
COL4A3
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Product Notes
The COL4A3 col4a3 (Catalog #AAA38053) is a Recombinant Protein produced from Sf9 insect cells. and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Collagen Type IV Alpha 3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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