COVID 19 Spike protein, Omicron variant, B.1.1.529, 21K clade Coronavirus Recombinant Protein | COVID-19 recombinant protein

Recombinant SARS-CoV-2 Spike protein, Omicron variant, B.1.1.529, 21K clade

Cat. Num. AAA47148

• Synonyms: COVID 19 Spike protein, Omicron variant, B.1.1.529, 21K clade Coronavirus; N/A; Recombinant SARS-CoV-2 Spike protein, Omicron variant, B.1.1.529, 21K clade; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike protein; Omicron variant; B.1.1.529; 21K clade; Omicron mutant; Omicron variant of concern Spike glycoprotein; S protein; COVID-19 recombinant protein

• Host: HEK293
• Form/Format: PBS 20% Glycerol
• Sequence Positions: 16-1211

• Tag Information: 8xHis at the C terminus
• Endotoxins: Less than 0.1 ng/ug (1 IEU/ug), as measured by LAL method.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: The protein should be stored at -20°C to -70°C preferably in small aliquots to avoid repeated freeze-thaw cycles. Stability: At least 12 months at -20°C to -70°C and at least 1 month at 2°C to 8°C.

Functional Data

(Binding curve of anti-Cov-19&SARS S glycoprotein antibody IgG CR3022 to the trimeric Spike Omicron variant of concern)

SDS-PAGE

Sequence

Related Product Information for COVID-19 recombinant protein

The first reports of atypical pneumonia in China were announced at the very end of 2019, in the city of Wuhan, Hubei province. Its cause was identified in January 2020 as a novel ?-CoV (1), named SARS- CoV-2, which started spreading very quickly causing a global pandemic COVID-19. Spike protein attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (2, 3). The protein forms a trimer in vivo mediating receptor binding and membrane fusion (4). It has been demonstrated by Florian Krammer’s group that certain mutations and the inclusion of trimerization motif can stabilize recombinant Spike protein (5, 6).

On November 24, 2021, South Africa reported the identification of a new SARS-CoV-2 variant, B.1.1.529, to the World Health Organization (WHO). B.1.1.529 was first detected in specimens collected on November 11, 2021 in Botswana and on November 14, 2021 in South Africa. Omicron has many concerning spike protein substitutions, some of which are known from other variants to be associated with reduced susceptibility to available monoclonal antibody therapeutics or reduced neutralization by convalescent and vaccinee sera (7).

References

1. Zhu N., Zhang D., Wang W., et al. China Novel Coronavirus Investigating and Research Team. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med., 2020;382:727–733.
2. Hoffmann M., Kleine-Weber H., Schroeder S., et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 2020;181(2):271-280.
3. Wrapp D., Wang N., Corbett KS., et al. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science, 2020;367(6483):1260-1263.
4. Yin HS., Wen X., Paterson RG., Lamb RA., Jardetzky TS. Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation. Nature, 2006;439(7072):38-44.
5. Amanat, F., Stadlbauer, D., Strohmeier, S., et al. A serological assay to detect SARS-CoV-2 seroconversion in humans. Nat Med., 2020;26:1033–1036.
6. Stadlbauer, D., Amanat, F., Chromikova, V., et al. SARS?CoV?2 seroconversion in humans: A detailed protocol for a serological assay, antigen production, and test setup. Current Protocols in Microbiology, 2020;57, e100

NCBI and Uniprot Product Information

• UniProt Accession #: P0DTC2
• Molecular Weight: 137 kDa. Estimated Molecular Weight, SDS-PAGE: ~190 kDa