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product-image-AAA78555_AD13.jpg Application Data (Figure 2: Binding assay of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein with recombinant Human ACE2 Fc Protein. An ELISA plate was coated with 1ug/ml of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein, and the plate was incubated overnight at 4 degree C. Different concentrations (100 ng/well to 0 ng/well) of recombinant Human ACE2 Fc Protein was added to the wells. HRP conjugated goat anti-human Fc was used as secondary antibody.)

COVID 19 Spike S1 Coronavirus Recombinant Protein | COVID-19 recombinant protein

SARS CoV2 Spike S1 C-Term His Tag Protein

Applications
ELISA, Functional Assay
Purity
>95% by SDS-PAGE
Synonyms
COVID 19 Spike S1 Coronavirus; N/A; SARS CoV2 Spike S1 C-Term His Tag Protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S1; COVID-19 recombinant protein
Ordering
Host
CHO-K1
Purity/Purification
>95% by SDS-PAGE
Form/Format
PBS and 10% Glycerol
Sequence Positions
14-683aa
Applicable Applications for COVID-19 recombinant protein
ELISA, FA (Functional Assay)
Preparation and Storage
It is shipped on ice packs. Upon arrival, store at -20 degree C. Do not freeze-thaw multiple times.

Application Data

(Figure 2: Binding assay of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein with recombinant Human ACE2 Fc Protein. An ELISA plate was coated with 1ug/ml of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein, and the plate was incubated overnight at 4 degree C. Different concentrations (100 ng/well to 0 ng/well) of recombinant Human ACE2 Fc Protein was added to the wells. HRP conjugated goat anti-human Fc was used as secondary antibody.)

product-image-AAA78555_AD13.jpg Application Data (Figure 2: Binding assay of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein with recombinant Human ACE2 Fc Protein. An ELISA plate was coated with 1ug/ml of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein, and the plate was incubated overnight at 4 degree C. Different concentrations (100 ng/well to 0 ng/well) of recombinant Human ACE2 Fc Protein was added to the wells. HRP conjugated goat anti-human Fc was used as secondary antibody.)

SDS-PAGE

(Figure-1: SDS-PAGE analysis of purified SARS-CoV-2 Spike S1-His recombinant protein. 0.5, 1.0 and 2.0ug protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)

product-image-AAA78555_SDS_PAGE15.jpg SDS-PAGE (Figure-1: SDS-PAGE analysis of purified SARS-CoV-2 Spike S1-His recombinant protein. 0.5, 1.0 and 2.0ug protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)
Related Product Information for COVID-19 recombinant protein
The target protein is expressed with sequence (AA 14-683) of SARS-CoV-2/COVID-19 Spike S1 sequence fused with a 6×His tag in C-Terminal.
The spike protein (S1) of coronavirus (CoV2) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Categories/Family for COVID-19 recombinant protein

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Product Notes

The COVID-19 (Catalog #AAA78555) is a Recombinant Protein produced from CHO-K1 and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 14-683aa. AAA Biotech's COVID 19 Spike S1 Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA, FA (Functional Assay). Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike S1 Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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