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Application Data
(Loaded ACE2 Protein, Human, Recombinant (mFc Tag)(Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.)
COVID 19 Spike S1 Coronavirus Recombinant Protein | COVID-19 recombinant protein
SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified)
Purity
>90% as determined by SDS-PAGE. >95% as determined by SEC-HPLC.
Synonyms
COVID 19 Spike S1 Coronavirus; N/A; SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S1; COVID-19 recombinant protein
Host
HEK293 Cells
Purity/Purification
>90% as determined by SDS-PAGE. >95% as determined by SEC-HPLC.
Form/Format
Lyophilized from sterile PBS, pH7.4
Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Sequence
Val16-Arg685
Species
SARS-CoV-2
Endotoxin
<1.0 EU per ug protein as determined by the LAL method.
Predicted N Terminal
Val16
Tag
C-His
Protein Construction
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1)(Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus.
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2ug/mL (100uL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag)(40591-V08H), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag)(Cat: 40591-V08H) is 200-600ng/mL.2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag)(Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.
Preparation and Storage
Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Application Data
(Loaded ACE2 Protein, Human, Recombinant (mFc Tag)(Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.)
Application Data
(Loaded ACE2 Protein, Human, Recombinant (mFc Tag)(Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.)
Application Data
Application Data
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 10ug/mL (100uL/well) can bind 2019-nCoV Spike Protein (S1, His tag)(40591-V08H), the EC50 of 2019-nCoV Spike Protein (S1, His tag)(40591-V08H) is 200-400ng/mL.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 10ug/mL (100uL/well) can bind 2019-nCoV Spike Protein (S1, His tag)(40591-V08H), the EC50 of 2019-nCoV Spike Protein (S1, His tag)(40591-V08H) is 200-400ng/mL.)
Application Data
Application Data
NCBI and Uniprot Product Information
Molecular Weight
51.5 kDa
Similar Products
Product Notes
The COVID-19 (Catalog #AAA258309) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Val16-Arg6 85. It is sometimes possible for the material contained within the vial of "COVID 19 Spike S1 Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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