Carboxypeptidase B2 / CPB2 Recombinant Protein | CPB2 recombinant protein

Recombinant Human Carboxypeptidase B2 / CPB2 Protein (His tag)

Cat. Num. AAA173544

• Gene Names: CPB2; CPU; PCPB; TAFI
• Purity: > 97 % as determined by reducing SDS-PAGE.
• Synonyms: Carboxypeptidase B2 / CPB2; N/A; Recombinant Human Carboxypeptidase B2 / CPB2 Protein (His tag); Carboxypeptidase B2; Carboxypeptidase U; CPU; Plasma Carboxypeptidase B; pCPB; Thrombin-Activable Fibrinolysis Inhibitor; TAFI; CPB2; CPB2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 97 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80
• Sequence: Met 1-Val 423

• Species: Human
• Tag: C-His
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized protein should be stored at < -20°C.; Reconstituted protein solution can be stored at 4-7°C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20°C for 3 months.; This product is provided as lyophilized powder which is shipped with blue ice.

SDS-PAGE

Related Product Information for CPB2 recombinant protein

Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

NCBI and Uniprot Product Information

• NCBI GI #: 126273569
• NCBI GeneID: 1361
• NCBI Accession #: NP_001863.2
• NCBI GenBank Nucleotide #: NM_001872.3
• Molecular Weight: Mol Mass: 47.4 kDa; AP Mol Mass: 45-50 kDa
• NCBI Official Full Name: carboxypeptidase B2 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B2
• NCBI Official Symbol: CPB2
• NCBI Official Synonym Symbols: CPU; PCPB; TAFI
• NCBI Protein Information: carboxypeptidase B2
• UniProt Protein Name: Carboxypeptidase B2
• UniProt Gene Name: CPB2
• UniProt Synonym Gene Names: CPU; pCPB; TAFI