Standard Curve (Sample)
Human TAFI/CPB2 ELISA Kit | CPB2 elisa kit
Human TAFI/CPB2 PicoKine ELISA Kit
Gene Names
CPB2; CPU; PCPB; TAFI
Reactivity
Human
Synonyms
TAFI/CPB2; N/A; Human TAFI/CPB2 PicoKine ELISA Kit; Carboxypeptidase U; CarboxypeptidaseU; CarboxypeptidaseB2; CP B2; CPB 2; CPB2; CPU; PCPB; Plasma carboxypeptidase B; Plasma carboxypeptidase B2; Procarboxypeptidase B; Procarboxypeptidase R; Procarboxypeptidase U; TAFI; Q96IY4 ; Carboxypeptidase B2; CPB2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CPB2
Sequence Length
386
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<15 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CPB2 elisa kit
Principle of the Assay: The Picokine™ Human CPB2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CPB2 with a 96-well strip plate that is pre-coated with antibody specific for CPB2. The detection antibody is a biotinylated antibody specific for CPB2. The kit contains recombinant Human CPB2 with immunogen: Expression system for standard: NS0; Immunogen sequence: M1-V423. The kit is analytically validated with ready-to-use reagents. To measure Human CPB2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CPB2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CPB2 in the sample.
Background/Introduction: Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
Background/Introduction: Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,922 Da
NCBI Official Full Name
carboxypeptidase B2 isoform 2 preproprotein
NCBI Official Synonym Full Names
carboxypeptidase B2
NCBI Official Symbol
CPB2
NCBI Official Synonym Symbols
CPU; PCPB; TAFI
NCBI Protein Information
carboxypeptidase B2
UniProt Protein Name
Carboxypeptidase B2
UniProt Gene Name
CPB2
UniProt Synonym Gene Names
CPU; pCPB; TAFI
UniProt Entry Name
CBPB2_HUMAN
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Product Notes
The Human CPB2 cpb2 (Catalog #AAA11676) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11676 ELISA Kit recognizes Human CPB2. It is sometimes possible for the material contained within the vial of "TAFI/CPB2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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