Standard Curve (Sample)
Rat colony stimulating factor 1 (macrophage) ELISA Kit | CSF1 elisa kit
Rat Macrophage Colony-Stimulating Factor, M-CSF ELISA Kit
Reactivity
Rat
Synonyms
colony stimulating factor 1 (macrophage); N/A; Rat Macrophage Colony-Stimulating Factor, M-CSF ELISA Kit; Rat Macrophage Colony-Stimulating Factor; M-CSF ELISA kit; MCSF; MGC31930; OTTHUMP00000013363; OTTHUMP00000013364; colony stimulating factor 1; macrophage colony stimulating factor; CSF1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat CSF1. No significant cross-reactivity or interference between Rat CSF1 and analogues was observed.
Sequence Length
566
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CSF1 elisa kit
For SampleFor the quantitative determination of rat macrophage colony-stimulating factor (M-CSF) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for M-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any M-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for M-CSF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of M-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for M-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any M-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for M-CSF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of M-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
62,187 Da
NCBI Official Full Name
Macrophage colony-stimulating factor 1
NCBI Official Synonym Full Names
colony stimulating factor 1 (macrophage)
NCBI Official Symbol
Csf1
NCBI Protein Information
macrophage colony-stimulating factor 1; MCSF; CSF-1
UniProt Protein Name
Macrophage colony-stimulating factor 1
UniProt Gene Name
Csf1
UniProt Synonym Gene Names
Csfm; CSF-1; MCSF
UniProt Entry Name
CSF1_RAT
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Product Notes
The Rat CSF1 csf1 (Catalog #AAA15055) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA15055 ELISA Kit recognizes Rat CSF1. It is sometimes possible for the material contained within the vial of "colony stimulating factor 1 (macrophage), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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