Macrophage Colony stimulating Factor Active Protein | Csf1 active protein

Mouse Macrophage Colony stimulating Factor Recombinant

Cat. Num. AAA76388

• Gene Names: Csf1; op; Csfm; MCSF; C87615
• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Macrophage Colony stimulating Factor; N/A; Mouse Macrophage Colony stimulating Factor Recombinant; Csf1 active protein

• Host: E coli (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant mouse MCSF was lyophilized from a 0.2 um filtered PBS solution.

• Source: Optimized DNA sequence encoding mouse M-CSF extracellular domain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Biological Activity: The ED(50) was determined by the dose-dependent stimulation of the proliferation of of M-NFS-60 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
• Method: Cell activation assay and immunodetection of cytokinesSingle spleen cell suspensions were prepared by mechanical disruption on a cell strainer (70 um pore diameter,,) in Dulbecco's D-PBS.; Red blood cells were lysed using Hemolytic Gey's Solution as previously describedOsteoclastogenesis assaysOsteoclastogenesis assays were performed as in 5 cells were plated in each well of a 24-well dish with 60 ng/mL RANKL for 3 days.; After 3 days, 2×103 MDA-231, shControl, or shEGFR-MDA-231 cells were plated with the bone marrow cells, with 60 ng/mL RANKL and 10 ng/mL MCSF and grown for 3 days before TRAP staining.; For TRAP staining, cells were washed with 1×PBS, fixed with ice cold methanol for 10 minutes, and stained in fresh TRAP solution for 15 minutes at 37 degree C.; TRAP solution was replaced with 1×PBS for cell counting under the microscope.Generation of bone marrow-derived macrophagesBone marrow-derived macrophages (BMDM) were generated by flushing the bone marrow from the femurs of BALB/c mice with RPMI 1640 media.; Freshly collected bone marrow cells were cultured overnight in complete media (CM; RPMI 1640, 10% fetal bovine serum; [Atlanta, ], 10 mM HEPES buffer, 10 mM nonessential amino acids, 10 mM sodium pyruvate) containing 5 ng/ml recombinant murine M-CSF.; The non-adherent cells were then collected and cultured for an additional six days in CM with 30 ng/ml M-CSF to generate macrophages.; Induction of 2nd iPSCsAll isolated somatic cells were cultured in the presence of Dox (2 ug/ml) for induction of 2nd iPSCs.; Fibroblasts and hematopoietic cells were cultured in ES/iPSC medium.; FLCD45 were cultured in the presence of 10 ng/ml human TPO, 10 ng/ml mouse EPO, 10 ng/ml mouse IL-3, 10 ng/ml mouse IL-6, 10 ng/ml mouse Flt3 ligand, 10 ng/ml mouse GM-CSF, 10 ng/ml mouse VEGF and 50 ng/ml mouse SCF.; HSCs, HPCs and MPs were cultured in the presence of 10 ng/ml human TPO, 10 ng/ml mouse IL-3, 10 ng/ml mouse IL-6 and 10 ng/ml mouse Flt3 ligand.; Macrophages were cultured in the presence of 5 ng/ml M-CSF.
• Molecular Function: Cytokine, Growth-factor
• Biological Process: Immunity, Inflammatory-response
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

(TcREG inhibit osteoclast activity in an antigen- and contact-independent manner by secreted cytokines.Cells were re-fed with medium containing M-CSF and RANKL every 2 to 3 days.)

Application Data

(Pro-resorptive cytokine expression and osteoclastogenesis in the synovial fluid of patients with gouty arthritis.PBMCs, SFMCs, and T cell-depleted SFMCs (3 × 105 cells/well) were cultured for 10 days in the presence of macrophage colony-stimulating factor (M-CSF; 30 ng/ml) at the different RANKL concentrations and then were stained for tartrate-resistant acid phosphatase .)

Application Data

Application Data

(PLC?2 is required for in vitro osteoclast development.(a) Representative TRAP-stained images of wild-type and PLC?2?/? bone marrow cells cultured in the presence of the indicated concentrations of recombinant murine M-CSF and RANKL for 4 days.)

Application Data

(Lung-derived prominin-1.Exposure of prominin-1+ cells to M-CSF for 7 days resulted in formation of E.coli phagocytising F4/80-positive cells .)

Product Categories/Family for Csf1 active protein

PDGF family; PDGF family; Recombinant Mouse Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 166064046
• NCBI GeneID: 12977
• NCBI Accession #: NP_001107001.1
• NCBI GenBank Nucleotide #: NM_001113529.1
• UniProt Accession #: P07141; P03228; P09581 CSF1R_MOUSE; P46414; P07333 CSF1R_HUMAN
• Molecular Weight: 29,353 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 isoform 2
• NCBI Official Synonym Full Names: colony stimulating factor 1 (macrophage)
• NCBI Official Symbol: Csf1
• NCBI Official Synonym Symbols: op; Csfm; MCSF; C87615
• NCBI Protein Information: macrophage colony-stimulating factor 1; osteopetrosis
• UniProt Protein Name: Macrophage colony-stimulating factor 1
• UniProt Gene Name: Csf1
• UniProt Synonym Gene Names: Csfm; CSF-1; MCSF
• UniProt Entry Name: CSF1_MOUSE