Casein kinase I isoform epsilon Recombinant Protein | CSNK1E recombinant protein

Recombinant Human Casein kinase I isoform epsilon protein

Cat. Num. AAA81600

• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Casein kinase I isoform epsilon; N/A; Recombinant Human Casein kinase I isoform epsilon protein; CSNK1E recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Liquid or Lyophilized powder; If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8
• Sequence Positions: 17-231aa; Partial
• Sequence: SGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEK

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for CSNK1E recombinant protein

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phospohorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.

Product Categories/Family for CSNK1E recombinant protein

Signal Transduction

References

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Biol. Chem. 278:14066-14073(2003) SCFbeta-TRCP controls clock-dependent transcription via casein kinase 1-dependent degradation of the mammalian period-1 (Per1) protein.Shirogane T., Jin J., Ang X.L., Harper J.W.J. Biol. Chem. 280:26863-26872(2005) Dysbindin structural homologue CK1BP is an isoform-selective binding partner of human casein kinase-1.Yin H., Laguna K.A., Li G., Kuret J.Biochemistry 45:5297-5308(2006) Negative regulation of LRP6 function by casein kinase I epsilon phosphorylation.Swiatek W., Kang H., Garcia B.A., Shabanowitz J., Coombs G.S., Hunt D.F., Virshup D.M.J. Biol. Chem. 281:12233-12241(2006) Posttranslational regulation of the mammalian circadian clock by cryptochrome and protein phosphatase 5.Partch C.L., Shields K.F., Thompson C.L., Selby C.P., Sancar A.Proc. Natl. Acad. Sci. U.S.A. 103:10467-10472(2006) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.Mol. Cell 31:438-448(2008) A quantitative atlas of mitotic phosphorylation.Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.Anal. Chem. 81:4493-4501(2009) Large-scale proteomics analysis of the human kinome.Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.Mol. Cell. Proteomics 8:1751-1764(2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.Sci. Signal. 2:RA46-RA46(2009) Role of CK1 in GSK3beta-mediated phosphorylation and degradation of snail.Xu Y., Lee S.H., Kim H.S., Kim N.H., Piao S., Park S.H., Jung Y.S., Yook J.I., Park B.J., Ha N.C.Oncogene 29:3124-3133(2010) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.Sci. 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NCBI and Uniprot Product Information

• NCBI GI #: 583966087
• NCBI GeneID: 102800317
• NCBI Accession #: NP_001276841.1
• NCBI GenBank Nucleotide #: NM_001289912.1
• UniProt Accession #: P49674
• Molecular Weight: 51.5 kDa
• NCBI Official Full Name: casein kinase I isoform epsilon
• UniProt Protein Name: Casein kinase I isoform epsilon
• UniProt Gene Name: CSNK1E
• UniProt Synonym Gene Names: CKI-epsilon; CKIe
• UniProt Entry Name: KC1E_HUMAN