WB (Western Blot)
(Anti-CSNK1E (E346) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human CSNK1E Polyclonal Antibody | anti-CSNK1E antibody
CSNK1E Antibody (C-term)
Gene Names
CSNK1E; HCKIE; CKIepsilon
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CSNK1E, Antibody; CSNK1E Antibody (C-term); Casein kinase I isoform epsilon; CKI-epsilon; CKIe; CSNK1E; anti-CSNK1E antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CSNK1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the C-terminal region of human CSNK1E.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
331-360
Sequence Length
416
Applicable Applications for anti-CSNK1E antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Casein kinases are operationally defined bytheir preferential utilization of acidicproteins such as caseins as substrates. Canphosphorylate a large number of proteins.Participates in Wnt signaling.Phosphorylates DVL1 and DVL2. Centralcomponent of the circadian clock. Inbalance with PP1, determines the circadianperiod length, through the regulation of thespeed and rhythmicity of PER1 and PER2phosphorylation. Controls PER1 and PER2nuclear transport and degradation. Inhibitscytokine-induced granuloytic differentiation.
Cellular Location
Cytoplasm. Nucleus.
Tissue Location
Expressed in all tissues examined, includingbrain, heart, lung, liver, pancreas, kidney,placenta and skeletal muscle Expressed inmonocytes and lymphocytes but not ingranulocytes
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-CSNK1E (E346) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CSNK1E (E346) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of anti-CK1e C-term Pab in SKBR3 cell lysate. CK1e (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of anti-CK1e C-term Pab in SKBR3 cell lysate. CK1e (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-CSNK1E antibody
CK1e is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. This protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. It has been shown to phosphorylate period, a circadian rhythm protein.
Product Categories/Family for anti-CSNK1E antibody
References
Hino, S., et al., J. Biol. Chem. 278(16):14066-14073 (2003). Eide, E.J., et al., J. Biol. Chem. 277(19):17248-17254 (2002). Keesler, G.A., et al., Neuroreport 11(5):951-955 (2000). Cegielska, A., et al., J. Biol. Chem. 273(3):1357-1364 (1998). Kloss, B., et al., Cell 94(1):97-107 (1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
casein kinase I isoform epsilon
NCBI Official Synonym Full Names
casein kinase 1, epsilon
NCBI Official Symbol
CSNK1E
NCBI Official Synonym Symbols
HCKIE; CKIepsilon
NCBI Protein Information
casein kinase I isoform epsilon
UniProt Protein Name
Casein kinase I isoform epsilon
UniProt Gene Name
CSNK1E
UniProt Synonym Gene Names
CKI-epsilon; CKIe
UniProt Entry Name
KC1E_HUMAN
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Product Notes
The CSNK1E csnk1e (Catalog #AAA287986) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 331-360. The CSNK1E Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CSNK1E can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CSNK1E csnk1e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSNK1E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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