Canine Connective Tissue Growth Factor ELISA Kit | CTGF elisa kit

Canine Connective Tissue Growth Factor ELISA Kit

Cat. Num. AAA92444

• Gene Names: CTGF; CCN2; NOV2; HCS24; IGFBP8
• Reactivity: Canine
• Synonyms: Connective Tissue Growth Factor; N/A; Canine Connective Tissue Growth Factor ELISA Kit; CTGF elisa kit

• Reactivity: Canine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CTGF elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This CTGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CTGF. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY CTGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTGF antibody and an CTGF-HRP conjugate. The assay sample and buffer are incubated together with CTGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTGF concentration since CTGF from samples and CTGF-HRP conjugate compete for the anti-CTGF antibody binding site. Since the number of sites is limited, as more sites are occupied by CTGF from the sample, fewer sites are left to bind CTGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTGF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CTGF elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4503123
• NCBI GeneID: 1490
• NCBI Accession #: NP_001892.1
• NCBI GenBank Nucleotide #: NM_001901.2
• Molecular Weight: 35,205 Da
• NCBI Official Full Name: connective tissue growth factor
• NCBI Official Synonym Full Names: connective tissue growth factor
• NCBI Official Symbol: CTGF
• NCBI Official Synonym Symbols: CCN2; NOV2; HCS24; IGFBP8
• NCBI Protein Information: connective tissue growth factor; IBP-8; IGFBP-8; CCN family member 2; IGF-binding protein 8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
• UniProt Protein Name: Connective tissue growth factor
• UniProt Gene Name: CTGF
• UniProt Synonym Gene Names: CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8
• UniProt Entry Name: CTGF_HUMAN